catalog number :
MBS440117
products full name :
GAPDH (C17) Antibody
products short name :
GAPDH
other names :
Glyceraldehyde-3-phosphate dehydrogenase; Glyceraldehyde-3-phosphate dehydrogenase; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; peptidyl-cysteine S-nitrosylase GAPDH; glyceraldehyde-3-phosphate dehydrogenase; 38 kDa BFA-dependent ADP-ribosylation substrate; BARS-38; Peptidyl-cysteine S-nitrosylase GAPDH (EC:2.6.99.-)
products gene name :
GAPDH
other gene names :
Gapdh; Gapdh; Gapd; BARS-38; Gapd; GAPDH
uniprot entry name :
G3P_RAT
reactivity :
Mouse, rat, human, rabbit, chicken, dog, pig, cat
specificity :
Recognizes mouse, rat, human, rabbit, chicken, dog, pig, and cat GAPDH by western blotting.
form :
200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.
concentration :
0.200 mg/ml
storage stability :
Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.
tested application :
Western Blot (WB)
app notes :
Recommended western blotting starting dilution 1:400.
other info1 :
Origin: GAPDH (C17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping near the carboxy terminus of human GAPDH.
other info2 :
Immunogen: A synthetic peptide mapping near the carboxy terminus of human GAPDH.
products description :
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is well known for its glycolytic function of converting D-glyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate. In more recent studies GAPDH has been shown to be involved in membrane fusion, microtubule bundling, phosphotransferase activity, nuclear RNA export, prostate cancer progression, programmed neuronal cell death, DNA replication, and DNA repair. GAPDH has a molecular mass of 37 kDa and its expression is upregulated in prostate, liver, and lung cancers (1-3).
products references :
1.Meyer-Siegler K, Mauro DJ, Seal G, Wurzer J, deRiel JK, and Sirover MA. 1991. A human nuclear uracil DNA glycosylase is the 37-kDa subunit of glcyeraldehyde-3-phosphate dehydrogenase. PNAS USA 88: 8460-8464.2.Rondinelli RH, Epner DE, and Tricoli JV. 1997. Increased glyceraldehydes-3-phosphate dehydrogenase gene expression in late pathological stage human prostate cancer. Prostate Cancer Prostatic Dis. 2: 66-72.3.Tarbe N, Evtimova V, Burtscher H, Jarsch M, Alves F, and Weidle UH. 2001. Transcriptional profiling of cell lines derived from an orthotopic pancreatic tumor model reveals metastasis-associated genes. Anticancer Res. 5: 3221-3228.
ncbi mol weight :
35,828 Da
ncbi pathways :
Alzheimer's Disease Pathway 83489!!Alzheimer's Disease Pathway 509!!Androgen Receptor Signaling Pathway 198529!!Biosynthesis Of Amino Acids Pathway 791250!!Biosynthesis Of Amino Acids Pathway 795174!!Biosynthesis Of Antibiotics Pathway 1146234!!Carbon Metabolism Pathway 816129!!Carbon Metabolism Pathway 817567!!Gluconeogenesis Pathway 1178045!!Gluconeogenesis, Oxaloacetate = Fructose-6P Pathway 434661
ncbi summary :
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. A similar protein in human and mouse has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The encoded protein was originally identified as a key glycolytic enzyme that converts D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Subsequent studies in human and mouse have assigned a variety of additional functions to the protein including nitrosylation of nuclear proteins. Many pseudogenes similar to this locus are found throughout the rat genome. [provided by RefSeq, Jan 2014]