antibody

S-tag (E15)+HRP Antibody

MBS440104

0.2 mg

285 USD

polyclonal

rabbit

HRP

western blot, ELISA, immunohistochemistry, enzyme immunoassay

Antibody

S-tag (E15)+HRP Antibody

S-tag+HRP

S-tag+HRP

Detects proteins with the S-tag, KETAAAKFERQHMDS

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

0.200 mg/ml

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB),ELISA (EIA), Immunohistochemistry (IHC)

The suggested western blotting starting dilution is at 1:1000.

Immunogen: Synthetic peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A.

S-tag is an epitope tag composed of a 15 residue peptide, KETAAAKFERQHMDS, derived from the pancreatic ribonuclease A. Frederick Richards discovered the S-peptide/S-protein system. Ribonuclease A is split by enzyme subtilisin between amino acids 20 and 21 yielding ribonuclease S. Ribonuclease S is composed of the peptide corresponding to the first 20 amino acids, S-peptide, attached to the remainding amino acids 21-124, S-protein. Ribonuclease S has the full enzymatic activity of the parent protein and the two components, S-peptide and S-protein could be separated by reversible denaturation. The separation abolished enzymatic activity, but when the two components were mixed together again, fully active ribonuclease S is formed. The unique property of reconstituting enzymatic activity by the S-tag peptide:S-protein interaction enables sensitive quantitative measurement of any fusion protein by a simple assay.

0.2 mg

285 USD