cyclin B1 (V152) Antibody | MyBioSource MBS440095 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

cyclin B1 (V152) Antibody

catalog :

MBS440095

quantity :

0.1 mg

price :

340 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunoprecipitation, flow cytometry, FACS, immunohistochemistry - paraffin section

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS440095

products type :

Antibody

products full name :

cyclin B1 (V152) Antibody

products short name :

cyclin B1

other names :

G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin-B1; G2/mitotic-specific cyclin B1; cyclin B1

other gene names :

CCNB1; CCNB1; CCNB; CCNB

uniprot entry name :

CCNB1_HUMAN

clonality :

Monoclonal

host :

Mouse

reactivity :

Mouse, hamster, human

sequence length :

433

specificity :

Reacts with cyclin B1 of mouse, hamster and human origin

form :

100 ug/ml of mouse monoclonal IgG1 in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

concentration :

0.100 mg/ml

storage stability :

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

tested application :

Western Blot (WB), Immunoprecipitation (IP), Flow Cytometry (FC/FACS), Immunohistochemistry (IHC) Paraffin

app notes :

Western blotting starting dilution: 1:200.

other info1 :

Origin: Cyclin B1 (V152) is provided as a mouse monoclonal IgG1 derived from the fusion Sp2 myeloma cells with spleen cells from a mouse immunized with a polyhistidine tagged hamster cyclin B1.

other info2 :

Immunogen: Polyhistidine tagged hamster cyclin B1

products description :

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).

products references :

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63. 7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

ncbi gi num :

14327896

ncbi acc num :

NP_114172.1

ncbi gb acc num :

NM_031966.3

uniprot acc num :

P14635

ncbi mol weight :

44,102 Da

ncbi pathways :

AMPK Signaling Pathway 198868!!APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Cyclin B Pathway 105832!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!ATM Signaling Pathway 920969!!Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105829!!Activation Of NIMA Kinases NEK9, NEK6, NEK7 Pathway 771571!!C-MYB Transcription Factor Network Pathway 138073!!Cell Cycle Pathway 530733!!Cell Cycle Checkpoints Pathway 105739

ncbi summary :

The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq, Jul 2008]

size :

0.1 mg

price :

340 USD

more info or order :

MyBioSource product webpage