cyclin A (E23.1) Antibody | MyBioSource MBS440093 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

cyclin A (E23.1) Antibody

catalog :

MBS440093

quantity :

0.1 mg

price :

340 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, cow

application :

western blot, immunohistochemistry, immunoprecipitation

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS440093

products type :

Antibody

products full name :

cyclin A (E23.1) Antibody

products short name :

cyclin A

other names :

cyclin-A2; Cyclin-A2; cyclin-A2; cyclin A2

other gene names :

CCNA2; CCNA2; CCN1; CCNA; CCN1; CCNA; Cyclin-A

uniprot entry name :

CCNA2_HUMAN

clonality :

Monoclonal

host :

Mouse

reactivity :

Mouse, bovine, human

sequence length :

432

specificity :

Reacts with cyclin A of murine, bovine, and human origin

form :

100 ug/ml of mouse monoclonal IgG2a in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

concentration :

0.100 mg/ml

storage stability :

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

tested application :

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

app notes :

Western blotting starting dilution: 1:100.

other info1 :

Origin: Cyclin A (E67.1) is provided as mouse monoclonal IgG2a derived from a mouse immunized with bovine cyclin A. The epitope for this antibody has been mapped to a region containing the sequence LDYP. This epitope maps within amino acids 144-148 of human cyclin A2.Cyclin As that contain this epitope: Cow cyclin A2, human cyclin A2, hamster cyclin A2, mouse cylin A2.Cyclin As that do not contain this epitope: Chicken cyclin A2, human cyclin A1, mouse cyclin A1

other info2 :

Immunogen: Bovine cyclin A

products description :

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Cyclin A regulates at least two cdks, Cdc2 p34 and Cdk2, and is believed to be necessary for progression through S phase and is active during the G2-M transition (1&3,4). Cyclin B1 also associates with Cdc2 p34 and this complex is requisite for the G2-M progression. The Cyclin B1/Cdc2 p34 complex is known as MPF, M-phase kinase, maturation-promoting factor, or M-phase specific histone kinase, is widely accepted as the trigger of mitosis in all organisms (5-9).

products references :

1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Brechot C. 1993. Oncogenic activation of cyclin A. Curr Opin Genet Dev 3(1): 11-18.4. Goldstone S, Pavey S, Forrest A, Sinnamon J, Gabrielli B. 2001. Cdc25-dependent activation of cyclin A/cdk2 is blocked in G2 phase arrested cells independently of ATM/ATR. Oncogene 20(8): 921-932.5. Liu D, Liao C, Wolgemuth DJ. 2000. A role for cyclin A1 in the activation of MPF and G2-M transition during meiosis of male germ cells in mice. Dev Biol 224(2): 338-400.6. Hoffman I, Clarke PR, Marcotte MJ, Karsenti E, Draetta G. 1993. Phosphorylation and activation of human cdc25-C by cdc2-cyclin B and its involvement in the self-amplification of MPF at mitosis. EMBO J 12(1): 53-63. 7. Kishimoto T, Okumura E. 1997. In vivo regulation of the entry into M-phase: initial activation and nuclear translocation of cyclin B/Cdc2. Prog Cell Cycle Res 3:241-249.8. Baratte B, Meijer L, Galaktionov K, Beach D. 1992. Screening for antimitotic compounds using the cdc25 tyrosine phosphatase, an activator of the mitosis-inducing p34cdc2/cyclin Bcdc13 protein kinase. Anticancer Res 12(3): 873-880.9. Thron CD. 1994. Theoretical dynamics of the cyclin B-MPF system: a possible role for p13suc1. Biosystems 32(2):97-109.

ncbi gi num :

4502613

ncbi acc num :

NP_001228.1

ncbi gb acc num :

NM_001237.3

uniprot acc num :

P20248

ncbi mol weight :

48,551 Da

ncbi pathways :

AMPK Signaling Pathway 198868!!AMPK Signaling Pathway 989139!!AMPK Signaling Pathway 992181!!APC/C-mediated Degradation Of Cell Cycle Proteins Pathway 105825!!APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105831!!APC:Cdc20 Mediated Degradation Of Cell Cycle Proteins Prior To Satisfation Of The Cell Cycle Checkpoint Pathway 1127549!!ATF-2 Transcription Factor Network Pathway 138006!!Activation Of APC/C And APC/C:Cdc20 Mediated Degradation Of Mitotic Proteins Pathway 105829!!B Cell Receptor Signaling Pathway 198909!!Cdc20:Phospho-APC/C Mediated Degradation Of Cyclin A Pathway 1127550

ncbi summary :

The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. This cyclin binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. [provided by RefSeq, Jul 2008]

size :

0.1 mg

price :

340 USD

more info or order :

MyBioSource product webpage