antibody

DBC2 (N15) Antibody

MBS440073

0.2 mg

240 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

Antibody

DBC2 (N15) Antibody

DBC2

DBC2; Rho-related BTB domain-containing protein 2; rho-related BTB domain-containing protein 2; deleted in breast cancer 2 gene protein; p83; Rho-related BTB domain containing 2; Deleted in breast cancer 2 gene protein; p83

DBC2

RHOBTB2; RHOBTB2; DBC2; DBC2; KIAA0717

RHBT2_HUMAN

Polyclonal

Rabbit

Mouse, rat, human

727

Mouse, rat, and human DBC2

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

0.200 mg/ml

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB)

Origin: DBC2 (N15) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminal domain of human DBC2.

Immunogen: Synthetic peptide mapping to the amino terminal domain of human DBC2

Representational difference analysis (RDA) was the technique used to map a region on human chromosome 8p21 that contained a homozygous deletion several of the breast cancer specimens studied (1). The mapping of this region led to the identification of a gene with somatic mutations in breast cancer cells (1). This expression of this gene DBC2 (deleted in breast cancer) was extinguished in about half of the breast and lung cancer specimens that were examined, making it a good tumor suppressor candidate (1). The functional analysis of DBC2 found that expression of DBC2 in breast cancer cells missing DBC2 transcripts resulted growth inhibition (1).

1.) Siripurapu V, Meth J, Kobayashi N, Hamaguchi M. 2005. DBC2 significantly influences cell-cycle, apoptosis, cytoskeleton and membrane-trafficking pathways. J Mol Biol. Feb 11;346(1):83-9.2.) Yoshihara T, Collado D, Hamaguchi M. 2007, Cyclin D1 down-regulation is essential for DBC2's tumor suppressor function. Biochem Biophys Res Commun. Jul 13;358(4):1076-9. 1. Hamaguchi M, Meth JL, von Klitzing C, Wei W, Esposito D, Rodgers L, Walsh T, Welcsh P, King MC, Wigler MH. 2002. DBC2, a candidate for a tumor suppressor gene involved in breast cancer. PNAS USA 99(21):13647-13652.

12667516

AAG61157.1

Q9BYZ6

83,469 Da

Rho GTPase Cycle Pathway (106503); Signal Transduction Pathway (477114); Signaling By Rho GTPases Pathway (106502); Ubiquitin Mediated Proteolysis Pathway (83056); Ubiquitin Mediated Proteolysis Pathway (466)

The protein encoded by this gene is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

RHOBTB2: is a small Rho GTPase and a candidate tumor suppressor. The encoded protein interacts with the cullin-3 protein, a ubiquitin E3 ligase necessary for mitotic cell division. This protein inhibits the growth and spread of some types of breast cancer. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]. Protein type: G protein; G protein, monomeric, Rho; G protein, monomeric. Chromosomal Location of Human Ortholog: 8p21.3. Cellular Component: plasma membrane; cytosol. Molecular Function: GTP binding. Biological Process: regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction

0.2 mg

240 USD