beta-Actin (A11) Antibody | MyBioSource MBS440061 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

beta-Actin (A11) Antibody

catalog :

MBS440061

quantity :

0.2 mg

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS440061

products type :

Antibody

products full name :

beta-Actin (A11) Antibody

products short name :

beta-Actin

other names :

actin, cytoplasmic 1; Actin, cytoplasmic 1; actin, cytoplasmic 1; PS1TP5-binding protein 1; beta cytoskeletal actin; actin, beta; Beta-actin

other gene names :

ACTB; ACTB; BRWS1; PS1TP5BP1

uniprot entry name :

ACTB_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Mouse, rat, human

sequence length :

375

specificity :

Mouse, rat, and human beta-Actin

form :

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

concentration :

0.200 mg/ml

storage stability :

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

tested application :

Western Blot (WB), Immunohistochemistry (IHC)

app notes :

Western blotting and Immunohistochemistry. Recommended western blotting starting dilution 1:200.

other info1 :

Origin: beta-Actin (A11) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminal domain of human beta-Actin.

other info2 :

Immunogen: A synthetic peptide mapping to the amino terminal domain of human beta-Actin.

products description :

All eukaryotic cell types express actin. The regulation of the actin cytoskeleton is tightly linked to vital biological properties such as polarity, motility, cell-cell contact, exocytosis and proliferation (1&2). While lower eukaryotes such as yeast, have only one actin gene; higher eukaryotes have several isoforms encoded by a family of genes (3&4). At least six types of actin are present in mammalian tissues and fall into three classes (5). Alpha-actin expression is limited to various types of muscle, whereas beta and gamma are the principle constituents of filaments in other tissues (6).

products references :

1.) Hoffrogge R, Mikkat S, Scharf C, Beyer S, Christoph H, Pahnke J, Mix E, Berth M, Uhrmacher A, Zubrzycki IZ, Miljan E, Volker U, Rolfs A. 2006. 2-DE proteome analysis of a proliferating and differentiating human neuronal stem cell line (ReNcell VM). Proteomics. Mar;6(6):1833-47. 2.) Dogan S, Hu X, Zhang Y, Maihle NJ, Grande JP, Cleary MP. 2007. Effects of high-fat diet and/or body weight on mammary tumor leptin and apoptosis signaling pathways in MMTV-TGF-alpha mice. Breast Cancer Res. 9(6):R91. 1.Fuller CL, Braciale VL and Samelson LE. 2003. All roads lead to actin: the intimate relationship between TCR signaling and the cytoskeleton. Immunol Rev. 191:220-236. 2.Giganti A and Friederich E. 2003. The actin cytoskeleton as a therapeutic target: state of the art and future directions. Prog Cell Cycle Res. 5:511-525. 3.Doolittle RF. 1995. The origins and evolution of eukaryotic proteins. Philos Trans Royal Soc London Biol Sci. 349(1329):235-240. 4.Maccioni RB and Cambiazo V. 1995. Role of microtubule-associated proteins in the control of microtubule assembly. Physiol Rev. 75(4):835-864. 5.Schutt CE, Rozycki MD, Myslik JC, Lindberg U. 1995. A discourse on modeling F-actin. J. Struct. Biol. 115:186-198. 6.Barkalow K and Hartwig JH. 1995. Actin cytoskeleton. Setting the pace of cell movement. Curr Biol. 5(9):1000-1002.

ncbi gi num :

4501885

ncbi acc num :

NP_001092.1

ncbi gb acc num :

NM_001101.3

uniprot acc num :

P60709

ncbi mol weight :

41,737 Da

ncbi pathways :

Adherens Junction Pathway (83070); Adherens Junction Pathway (481); Arrhythmogenic Right Ventricular Cardiomyopathy Pathway (672454); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (117293); Arrhythmogenic Right Ventricular Cardiomyopathy (ARVC) Pathway (116129); Axon Guidance Pathway (105688); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Chaperonin-mediated Protein Folding Pathway (106242); Chromatin Modifying Enzymes Pathway (1127682)

ncbi summary :

This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq, Jul 2008]

uniprot summary :

ACTB: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs. Component of the BAF complex, which includes at least actin (ACTB), ARID1A, ARID1B/BAF250, SMARCA2, SMARCA4/BRG1, ACTL6A/BAF53, ACTL6B/BAF53B, SMARCE1/BAF57 SMARCC1/BAF155, SMARCC2/BAF170, SMARCB1/SNF5/INI1, and one or more of SMARCD1/BAF60A, SMARCD2/BAF60B, or SMARCD3/BAF60C. In muscle cells, the BAF complex also contains DPF3. Found in a complex with XPO6, Ran, ACTB and PFN1. Component of the MLL5-L complex, at least composed of MLL5, STK38, PPP1CA, PPP1CB, PPP1CC, HCFC1, ACTB and OGT. Interacts with XPO6 and EMD. Interacts with ERBB2. Interacts with GCET2. Belongs to the actin family. Protein type: Cytoskeletal; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 7p22. Cellular Component: cortical cytoskeleton; extracellular space; NuA4 histone acetyltransferase complex; protein complex; focal adhesion; postsynaptic density; ribonucleoprotein complex; cytosol; nucleoplasm; cytoskeleton; membrane; axon; cytoplasm; nuclear chromatin; plasma membrane. Molecular Function: protein binding; nucleosomal DNA binding; structural constituent of cytoskeleton; Tat protein binding; kinesin binding; nitric-oxide synthase binding; protein kinase binding; ATP binding. Biological Process: axon guidance; intercellular junction assembly and maintenance; establishment and/or maintenance of chromatin architecture; protein folding; de novo posttranslational protein folding; retinal homeostasis; cellular protein metabolic process; substantia nigra development; ATP-dependent chromatin remodeling; ephrin receptor signaling pathway; innate immune response; vascular endothelial growth factor receptor signaling pathway; blood coagulation; cell motility. Disease: Dystonia, Juvenile-onset; Baraitser-winter Syndrome 1

size1 :

0.2 mg

price1 :

240 USD

more info or order :

MyBioSource product webpage