PCNA (C19) Antibody | MyBioSource MBS440048 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

antibody

product name :

PCNA (C19) Antibody

catalog :

MBS440048

quantity :

0.2 mg

price :

240 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunoprecipitation

product information

catalog number :

MBS440048

products type :

Antibody

products full name :

PCNA (C19) Antibody

products short name :

PCNA

other names :

Proliferating cell nuclear antigen; Proliferating cell nuclear antigen; proliferating cell nuclear antigen; DNA polymerase delta auxiliary protein; cyclin; proliferating cell nuclear antigen; Cyclin

products gene name :

PCNA

other gene names :

PCNA; PCNA; ATLD2; PCNA

uniprot entry name :

PCNA_HUMAN

clonality :

Polyclonal

host :

Rabbit

reactivity :

Mouse, rat, human

sequence length :

261

specificity :

Mouse, rat and human PCNA

form :

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

concentration :

0.200 mg/ml

storage stability :

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

tested application :

Western Blot (WB), Immunopreciptation (IP), Immunohistochemistry (IHC)

app notes :

Western blotting starting dilution 1:200.

other info1 :

Origin: PCNA (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of human PCNA.

other info2 :

Immunogen: Synthetic peptide mapping to the carboxy terminus of human PCNA.

products description :

The evolutionarily conserved protein PCNA, or cyclin, was first characterized as a DNA polymerase accessory protein. It functions as a DNA sliding clamp for DNA polymerase delta and epsilon, and is an key component of for eukaryotic chromosomal DNA replication (1-3). In more recent research PCNA has been linked to an increasing number of cellular functions, these include cell cycle regulation, DNA repair, Okazaki fragment joining, DNA methylation, and chromatin assembly (2,4, &5). In cell cycle regulation p21 interacts with PCNA, which inhibits DNA replication (2,5). It has also been described that high levels of PCNA in the presence of p53 will lead to DNA repair (4). PCNA is also a very useful marker of cellular proliferation as it is seen most prominently expressed during the S phase of the cell cycle (6).

products references :

1.) Vogetseder A, Palan T, Bacic D, Kaissling B, Le Hir M. 2006. Proximal tubular epithelial cells are generated by division of differentiated cells in the healthy kidney. Am J Physiol Cell Physiol. Sept. Epub. 1. Kelman Z. 1997. PCNA: structure, functions and interactions. Oncogene 14(6):629-40.2. Tsurimoto T. 1999. PCNA binding proteins. Front Biosci 4:D849-D858.3. Balajee A.S., Geard C.R. 2001. Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells. Nucleic Acid Res 29(6):1341-1351.4. Pauneshku T., Mittal S., Protic M., Oryhon J., Korolev S.V., Joachimiak A., Woloschak G.E. 2001. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol 77(10):1007-1021.5. Rossig L., Jadidi A.S., Urbich C., Badorff C., Zeiher A.M., Dimmeler S. 2001. Akt-dependent phosphorylation of p21(Cip1) regulates PCNA binding and proliferation of endothelial cells. Mol Cell Biol 21(16):5644-5657.6. Celis J.E., Madsen P., Nielsen S., Celis A. 1986. Nuclear patterns of cyclin (PCNA) antigen distribution subdivide S-phase in cultured cells?some applications of PCNA antibodies. Leuk Res 10(3):237-249.

ncbi gi num :

129694

ncbi acc num :

P12004.1

uniprot acc num :

P12004

ncbi mol weight :

28,769 Da

ncbi pathways :

BARD1 Signaling Events Pathway 137959!!BRCA1-associated Genome Surveillance Complex (BASC) Pathway 413428!!BRCA1-associated Genome Surveillance Complex (BASC) Pathway 890555!!Base Excision Repair Pathway 105838!!Base Excision Repair Pathway 83043!!Base Excision Repair Pathway 451!!Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Cell Cycle Pathway 198811!!Cell Cycle Pathway 83054

ncbi summary :

The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome. [provided by RefSeq, Jul 2008]

size :

0.2 mg

price :

240 USD