antibody

p107 (C17) Antibody

MBS440044

0.2 mg

240 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunoprecipitation

Antibody

p107 (C17) Antibody

p107

p107; Retinoblastoma-like protein 1; retinoblastoma-like protein 1; 107 kDa retinoblastoma-associated protein; cellular protein 107; retinoblastoma-like 1 (p107); retinoblastoma-like 1; 107 kDa retinoblastoma-associated protein; p107; pRb1

p107

RBL1; RBL1; PRB1; p107; CP107; p107

RBL1_HUMAN

Polyclonal

Rabbit

Mouse, rat, human

1068

Mouse, rat and human p107

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

0.200 mg/ml

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Western blotting starting dilution: 1:200

Origin: p107 (C17) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminal domain of human p107.

Immunogen: Synthetic peptide mapping to the carboxy terminal domain of human p107.

The pocket protein family consists of three structurally and functionally related proteins, Rb, p107, and p130 (1). This family of tumor suppressors function to regulate important cellular transcription factors, such as the E2F family (1,2). The E2F proteins regulate the expression of genes whose products are important for cell cycle progression. The inactivation Rb is catalyzed by CDK phosphorylation thereby releasing E2F during the G1-S phase cellular progression (3). Unchecked inactivation of Rb in G1 phase has been indicated as a universal mechanism underlying cellular transformation (4,5). While Rb has been the most studied among the pocket proteins, p107 and p130 have also been shown to be key regulators of E2F (6). Several studies have also provided evidence that p107/p130 provide different functions in E2F regulation than does Rb (6,7). Rb, p107, and p130 each contain a conserved 'A/B pocket', which is the target of several viral oncoproteins, namely SV40 large T-antigen and adenovirus E1A (8).

1. Halaban R. 1999. Melanoma cell autonomous growth: the Rb/E2F pathway. Cancer Metastasis Rev 18(3):333-343.2. Sidle A., Palaty C., Dirks P., Wiggan O., Kiess M., Gill R.M., Wong A.K., Hamel P.A. 1996. Activity of the retinoblastoma family proteins, pRB, p107, and p130, during cellular proliferation and differentiation. Crit Rev Biochem Mol Biol 31(3):237-271.3. Classon M., Dyson N. 2001. p107 and p130: versatile proteins with interesting pockets. Exp Cell Res 264(1):135-147.4. Hatakeyama M., Weinberg R.A. 1995. The role of RB in cell cycle control. Prog Cell Cycle Res 1:9-19.5. Nevins J.R. 2001. The Rb/E2F pathway and cancer. Hum Mol Genet 10(7):699-703.6. Hurford R.K., Cobrinik D., Lee M.H., Dyson N. 1997. pRB and p107/p130 are required for the regulated expression of different sets of E2F responsive genes. Genes Dev 11(11):1447-1463.7. Smith E.J., Leone G., Nevins J.R. 1998. Distinct mechanisms control the accumulation of the Rb-related p107 and p130 proteins during cell growth. Cell Growth Differ 9(4):297-303.8. Knudsen E.S., Wang J.Y. 1998. Hyperphosphorylated p107 and p130 bind to T-antigen: identification of a critical regulatory sequence present in RB but not in p107/p130. Oncogene 16(13):1655-1663.

292374

AAA02489.1

P28749

114,688 Da

Adipogenesis Pathway (198832); Cell Cycle Pathway (530733); Cell Cycle, Mitotic Pathway (105765); Cell Cycle Pathway (198811); Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Cell Cycle - G1/S Transition Pathway (1084768); Cell Cycle - G1/S Transition Pathway (1108231); Cyclin D Associated Events In G1 Pathway (105767); E2F Transcription Factor Network Pathway (137934)

The protein encoded by this gene is similar in sequence and possibly function to the product of the retinoblastoma 1 (RB1) gene. The RB1 gene product is a tumor suppressor protein that appears to be involved in cell cycle regulation, as it is phosphorylated in the S to M phase transition and is dephosphorylated in the G1 phase of the cell cycle. Both the RB1 protein and the product of this gene can form a complex with adenovirus E1A protein and SV40 large T-antigen, with the SV40 large T-antigen binding only to the unphosphorylated form of each protein. In addition, both proteins can inhibit the transcription of cell cycle genes containing E2F binding sites in their promoters. Due to the sequence and biochemical similarities with the RB1 protein, it is thought that the protein encoded by this gene may also be a tumor suppressor. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Rb-like 1: Key regulator of entry into cell division. Directly involved in heterochromatin formation by maintaining overall chromatin structure and, in particular, that of constitutive heterochromatin by stabilizing histone methylation. Recruits and targets histone methyltransferases SUV420H1 and SUV420H2, leading to epigenetic transcriptional repression. Controls histone H4 Lys-20 trimethylation. Probably acts as a transcription repressor by recruiting chromatin-modifying enzymes to promoters. Potent inhibitor of E2F-mediated trans-activation. Forms a complex with adenovirus E1A and with SV40 large T antigen. May bind and modulate functionally certain cellular proteins with which T and E1A compete for pocket binding. May act as a tumor suppressor. Belongs to the retinoblastoma protein (RB) family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Transcription, coactivator/corepressor; Tumor suppressor. Chromosomal Location of Human Ortholog: 20q11.2. Cellular Component: nucleoplasm; transcription factor complex; nucleus. Molecular Function: protein binding; transcription factor binding. Biological Process: transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; viral reproduction; regulation of cell cycle; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; regulation of lipid kinase activity; chromatin modification; mitotic cell cycle; negative regulation of transcription from RNA polymerase II promoter

0.2 mg

240 USD