antibody

cyclin E (C19) Antibody

MBS440015

0.2 mg

240 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat

western blot, immunohistochemistry, immunoprecipitation

Antibody

cyclin E (C19) Antibody

cyclin E

G1/S-specific cyclin-E1; G1/S-specific cyclin-E1; G1/S-specific cyclin-E1; cyclin E; cyclin E1

Ccne1; Ccne1; Ccne; CYCLE; Ccne

CCNE1_RAT

Polyclonal

Rabbit

Mouse, rat, human

411

Mouse, rat, and human cyclin E

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

0.200 mg/ml

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Western blotting starting dilution: 1:200.

Origin: Cyclin E (C19) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the carboxy terminus of rat cyclin E.

Immunogen: Synthetic peptide mapping to the carboxy terminus of rat cyclin E.

During each cell cycle cyclins undergo periodic accumulation and destruction. As key regulators of the cell cycle the cyclins control important transitions by activating Cdks (1,2). Early in the G1 phase of the cell cycle cyclin D1 induction is followed by cyclin E induction (3,4). This sequential progression is marked early on in G1by the activation of Cdk4 and in mid to late G1 by the activation of Cdk2 and the hyperphosphorylation of pRB (3,5). The final transition into S phase is thought to be dependent on the increased expression and association of cyclin E and Cdk2 (5,6).

1.) Song SY, Lee I, Park C, Lee H, Hahm JC, Kang WK. 2005. Neolignans from Saururus chinensis inhibit PC-3 prostate cancer cell growth via apoptosis and senescence-like mechanisms. Int J Mol Med. Oct;16(4):517-23. 1. Bandara LR, Adamczewski JP, Zamanian M, Poon RY, Hunt T, Thangue NB. 1992. Cyclin A recruits p33cdk2 to the cellular transcription factor DRTF1. J Cell Sci Suppl 16: 77-85.2. Desdouets C, Sobczak-Thepot J, Murphy M, Brechot C. 1995. Cyclin A: Function and expression during cell proliferation. Prog Cell Cycle Res 1:115-123.3. Musgrove EA, Sarcevic B, Sutherland RL. 1996. Inducible expression of cyclin D1 in T-47D human breast cancer cells is sufficient for Cdk2 activation and pRB hyperphosphorylation. J Cell Biochem 60(3): 363-378.4. Geng Y, Whorishey W, Park MY, Bronson RT, Medema RH, Li T, Weinberg RA, Sicinski P. 1999. Rescue of cyclin D1 deficiency by knockin cyclin E. Cell 97(6): 767-777.5. Prall OW, Sarcevic B, Musgrove EA, Watts CK, Sutherland RL. 1997. Estrogen-induced activation of Cdk4 and Cdk2 during G1-S phase progression is accompanied by increased cyclin D1 expression and decreased cyclin-dependent kinase inhibitor association with cyclin E-Cdk2. J Biol Chem 272(16): 10882-10894.6. Sauer K, Lehner CF. 1995. The role of cyclin E in the regulation of entry into S phase. Prog Cell Cycle Res 1: 125-139.

158187539

NP_001094291.1

NM_001100821.1

P39949

47,482 Da

ATM Pathway 198524!!Androgen Receptor Signaling Pathway 198529!!B Cell Receptor Signaling Pathway 198486!!Cell Cycle Pathway 1178393!!Cell Cycle Checkpoints Pathway 1178394!!Cell Cycle, Mitotic Pathway 1178413!!Cell Cycle Pathway 198428!!Cell Cycle Pathway 83446!!Cell Cycle Pathway 463!!Cell Cycle - G1/S Transition Pathway 1085057

a cyclin-like protein; may be involved in growth stimulation [RGD, Feb 2006]

0.2 mg

240 USD