antibody

caspase-3 (A20) Antibody

MBS440008

0.2 mg

240 USD

polyclonal

rabbit

nonconjugated

western blot, immunohistochemistry, immunoprecipitation

Antibody

caspase-3 (A20) Antibody

caspase-3

caspase-3 preproprotein; Caspase-3; caspase-3; CASP-3; CPP-32; PARP cleavage protease; SREBP cleavage activity 1; apopain; caspase 3, apoptosis-related cysteine protease; cysteine protease CPP32; procaspase3; protein Yama; caspase 3, apoptosis-related cysteine peptidase; Apopain; Cysteine protease CPP32; CPP-32

CASP3; CASP3; CPP32; SCA-1; CPP32B; CPP32; CASP-3; CPP-32; SCA-1

CASP3_HUMAN

Polyclonal

Rabbit

Human

277

Human caspase-3 precursor (also designated CPP32)

200 ug/ml rabbit polyclonal IgG in 1 ml PBS containing 0.1 % sodium azide and 0.2% gelatin.

0.200 mg/ml

Store this product at 4 degree C, do not freeze. The product is stable for one year from the date of shipment.

Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)

Origin: Caspase-3 (also known as CPP32) is provided as an affinity purified rabbit polyclonal antibody, raised against a peptide mapping to the amino terminus of human caspase-3 precursor.

Immunogen: Synthetic peptide mapping to the amino terminal domain of human caspase-3 prescursor.

Caspases is a family of mammalian proteases that regulate many of the morphological and biochemical features of apoptosis. The currently identified caspases can be divided into three groups: apoptotic initiators, apoptotic executioners, and inflammatory mediators (1,2). Caspase-2, 8, and 9 have been identified as apoptotic initiators (3,4). Caspase-2 (also designated Nedd-2/ICH-1) can be alternatively spliced to produce two distinct Caspase-2 mRNA species. One mRNA species encodes a protein of 435 amino acids in length and the mRNA splice variant encodes a 312 amino acid protein. The larger protein, Caspase-2L, in an over expressed state, leads to programmed cell death. Conversely the over expression of Caspase-2S protein, a truncated version of Caspase-2L, has been shown to suppress cell death induced by serum deprivation (5). The executioner caspases include caspase-3, 6 and 7. Of these three proteases Caspase-3 is considered to be the essential for the chromatin margination, DNA fragmentation, and nuclear collapse during apoptosis (6). Caspase-1 (also known as ICE) is considered to be a mediator of the inflammatory response by converting the inactive precursor of interleukin-1b to the 17 kDa proinflammatory cytokine, IL-1b (7,8).

1.) Kim HJ, Kang SY, Kim SJ, Kim SH, Kim TY. 2004. Potentiation of UVB-induced apoptosis by novel phytosphingosine derivative, tetraacetyl phytosphingosine in HaCaT cell and mouse skin. Apoptosis. Jul;9(4):449-56. 1. Edadah BA, Faden AI. 2000. Caspase pathways, neuronal apoptosis, and CNS injury. Jounal of Neurotauma 17(10): 811-8292. Salvesen GS, Dixit VM. 1999. Caspase activation: the induced-proximity model. Proc Natl Acad Sci USA 96(20): 10964-109673. Zhang W, He Q, Chan LL, Zhou F, El Naghy M, Thompson EB, Ansari NH. 2001. Involvment of caspases in 4-hydroxy-alkenal-induced apoptosis in human leukemic cells. Free Radic Biol Med 30(6): 699-7064. Kruidering M, Evan GI. 2000. Caspase-8 in apoptosis: the beginning of ?the end? ? IUBMB 50(2): 85-905. Wang L, Miura M, Bergeron L, Zhu H, Yuan J. 1994. Ich-1, an Ice/ced-3-related gene, encodes both positive and negative regulators of programmed cell death. Cell 78(5): 739-7506. Slee EA, Adrain C, Martin SJ. 2001. Executioner caspase-3, -6, and ?7 perform distinct, non-redundant roles during the demolition phase of apoptosis. J Biol Chem 276(10): 7320-73267. Kostura MJ, Tocci MJ, Limjuco G, Chin J, Cameron P, Hillman AG, Chartrain NA, Schmidt JA. 1989. Identification of a monocyte specific pre-interleukin 1b convertase activity. Proc Natl Acad Sci USA 86(14): 5227-52318. Wilson KP, Black JA, Thompson JA, Kim EE, Griffith JP, Navia MA, Murcko MA, Chambers SP, Aldape RA, Raybuck SA, Livingston DJ. 1994. Structure and mechanism of interleukin-1b converting enzyme. Nature 370(6487): 251-252

14790119

NP_004337.2

NM_004346.3

P42574

31,608 Da

AGE/RAGE Pathway (698754); Activation Of DNA Fragmentation Factor Pathway (105683); Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (105672); Alpha6-Beta4 Integrin Signaling Pathway (198807); Alzheimer's Disease Pathway (83097); Alzheimer's Disease Pathway (509); Alzheimers Disease Pathway (672448); Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Amyotrophic Lateral Sclerosis (ALS) Pathway (920975)

This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq, Jul 2008]

CASP3: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a 216-Asp- -Gly-217 bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. Triggers cell adhesion in sympathetic neurons through RET cleavage. Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Interacts with BIRC6/bruce. Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. Inhibited by isatin sulfonamides. Belongs to the peptidase C14A family. Protein type: EC 3.4.22.56; Apoptosis; Motility/polarity/chemotaxis; Protease. Chromosomal Location of Human Ortholog: 4q34. Cellular Component: nucleoplasm; plasma membrane; cytosol; nucleus. Molecular Function: peptidase activity; cyclin-dependent protein kinase inhibitor activity; protein binding; cysteine-type endopeptidase activity; aspartic-type endopeptidase activity. Biological Process: extracellular matrix organization and biogenesis; nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; negative regulation of activated T cell proliferation; heart development; negative regulation of B cell proliferation; regulation of caspase activity; proteolysis; neuron differentiation; extracellular matrix disassembly; sensory perception of sound; B cell homeostasis; positive regulation of neuron apoptosis; response to wounding; erythrocyte differentiation; T cell homeostasis; DNA fragmentation during apoptosis; response to UV; cell structure disassembly during apoptosis; release of cytochrome c from mitochondria; cell fate commitment; negative regulation of cyclin-dependent protein kinase activity; keratinocyte differentiation; neuron apoptosis; induction of apoptosis via death domain receptors; caspase activation via cytochrome c; platelet formation; response to DNA damage stimulus; induction of apoptosis by oxidative stress; negative regulation of apoptosis

0.2 mg

240 USD