CD15/FUT4 (Reed-Sternberg Cell Marker) | MyBioSource MBS439984 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD15/FUT4 (Reed-Sternberg Cell Marker)

catalog :

MBS439984

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

rabbit

conjugate :

nonconjugated

clone name :

[FUT4/1478R]

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Hodgkin's Lymphoma stained with CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R).

image 2 :

Immunofluorescence staining of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.

image 3 :

Flow Cytometric Analysis of U937 cells using CD15 Rabbit Recombinant Monoclonal Antibody (FUT4/1478R) followed by goat anti-rabbit IgG-CF488 (Blue); Isotype Control (Red).

product information

catalog number :

MBS439984

products type :

Antibody

products full name :

CD15/FUT4 (Reed-Sternberg Cell Marker)

products short name :

[CD15/FUT4]

products name syn :

[3 Fucosyl N Acetyl Lactosamine; Alpha (1, 3) Fucosyltransferase; Alpha 13 fucosyltransferase FucT; ELAM Ligand Fucosyltransferase; ELFT; FCT3A; Fuc-TIV; Fucosyltransferase 4 Alpha 1 3 Fucosyltransferase Myeloid Specific; Fucosyltransferase 4; Galactoside 3 L Fucosyltransferase; Lewis X; LeX; SSEA1; Stage Specific Embryonic Antigen 1]

other names :

[alpha-(1,3)-fucosyltransferase 4; Alpha-(1,3)-fucosyltransferase 4; alpha-(1,3)-fucosyltransferase 4; fucosyltransferase 4; ELAM-1 ligand fucosyltransferase; Fucosyltransferase 4; Fucosyltransferase IV; Fuc-TIV; FucT-IV; Galactoside 3-L-fucosyltransferase]

products gene name :

[CD15]

products gene name syn :

[FUT4]

other gene names :

[FUT4; FUT4; LeX; CD15; ELFT; FCT3A; FUTIV; SSEA-1; FUC-TIV; ELFT; FCT3A; Fuc-TIV; FucT-IV]

clonality :

Monoclonal

isotype :

IgG

clone :

[FUT4/1478R]

host :

Rabbit

reactivity :

Human, Mouse, Rat. Others not known.

sequence length :

530

specificity :

CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on >95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkins disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

form :

200ug/ml of Ab purified by Protein A Column. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20°C to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) (Formalin-fixed)

app notes :

Flow Cytometry (1-2ug/million cells);. Immunofluorescence (1-2ug/ml);. Immunohistochemistry (Formalin-fixed) (5-10ug/ml for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 minutes followed by cooling at RT for 20 minutes) . Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunofluorescence (IF)

image3 heading :

Flow Cytometry (FC/FACS)

other info1 :

Human Unigene: 654379. Human Gene Symbol: FUT4. Human Chromosome Location: 11q21

other info2 :

Immunogen: Recombinant human full-length CD15 protein. Cellular Localization: Cell Surface and granular paranuclear. Positive Control: U937 cells, Reed-Sternberg's cells in Hodgkin's lymphoma

products references :

Hakomori, S. 1992. LeX and related structures as adhesion molecules. Histochem. J. 24: 771-776.

ncbi gi num :

4503811

ncbi acc num :

NP_002024.1

ncbi gb acc num :

NM_002033.3

uniprot acc num :

P22083

ncbi mol weight :

~220kDa

ncbi pathways :

Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (82995); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (370); Metabolic Pathways (132956); Other Types Of O-glycan Biosynthesis Pathway (82978); Other Types Of O-glycan Biosynthesis Pathway (349)

ncbi summary :

The product of this gene transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It catalyzes the synthesis of the non-sialylated antigen, Lewis x (CD15). [provided by RefSeq, Jan 2009]

uniprot summary :

May catalyze alpha-1,3 glycosidic linkages involved in the expression of Lewis X/SSEA-1 and VIM-2 antigens.CautionIt is uncertain whether Met-1 or Met-126 is the initiator.

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage