Alkaline Phosphatase (Tissue-Nonspecific) | MyBioSource MBS439977 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Alkaline Phosphatase (Tissue-Nonspecific)

catalog :

MBS439977

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

clone name :

[ALPL/597]

reactivity :

human, cow

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

image 2 :

Formalin-fixed, paraffin-embedded human Testicular Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

image 3 :

Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

product information

catalog number :

MBS439977

products type :

Antibody

products full name :

Alkaline Phosphatase (Tissue-Nonspecific)

products short name :

[Alkaline Phosphatase]

products name syn :

[Alkaline phosphatase, germ cell; Alkaline phosphatase, testicular and thymus; Alkaline phosphatase liver/bone/kidney; Alkaline phosphatase, tissue-nonspecific; ALPG; ALPL; Germ cell alkaline phosphatase; Testicular and thymus alkaline phosphatase; Tissue non-specific alkaline phosphatase; Tissue nonspecific ALP (TNAP or TNSALP)]

other names :

[alkaline phosphatase, tissue-nonspecific isozyme isoform 1 preproprotein; Alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, tissue-nonspecific isozyme; alkaline phosphatase, liver/bone/kidney; Alkaline phosphatase liver/bone/kidney isozyme]

products gene name :

[ALPL]

other gene names :

[ALPL; ALPL; HOPS; TNAP; TNALP; APTNAP; TNSALP; AP-TNAP; AP-TNAP; TNSALP]

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[ALPL/597]

host :

Mouse

reactivity :

Human, Cow. Others not known.

sequence length :

524

specificity :

There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. The exact physiological function of the alkaline phosphatases is not known. A proposed function of this form of the enzyme is matrix mineralization; however, mice that lack a functional form of this enzyme show normal skeletal development. This enzyme has been linked directly to hypo-phosphatasia, a disorder that is characterized by hypercalcemia and includes skeletal defects. The character of this disorder can vary, however, depending on the specific mutation since this determines age of onset and severity of symptoms. Alternatively spliced transcript variants, which encode the same protein, have been identified for this gene.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide store at 2-8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

FC/FACS: 0.5-1ug/million cells. IF: 0.5-1ug/ml. IHC-F: 1:50-1:100 for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Immunohistochemistry (IHC)

image4 description :

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Alkaline Phosphatase Mouse Monoclonal Antibody (ALPL/597).

other info1 :

Immunogen: Recombinant human ALPL protein. Cellular Localization: Cell Surface

other info2 :

Hu-chromosome Location: 1p36.12. Positive Control: Intestine

products references :

Griffin, C.A., et al. 1987. Human placental and intestinal alkaline phosphatase genes map to 2q34-q37. Am. J. Hum. Genet. 41: 1025-1034. Moss, D.W. 1987. Diagnostic aspects of alkaline phosphatase and its isoenzymes. Clin. Biochem. 20: 225-230.

ncbi gi num :

116734717

ncbi acc num :

NP_000469.3

ncbi gb acc num :

NM_000478.5

uniprot acc num :

P05186

ncbi mol weight :

55kDa

ncbi pathways :

AGE/RAGE Pathway (698754); BDNF Signaling Pathway (712093); Endochondral Ossification Pathway (198812); Folate Biosynthesis Pathway (83018); Folate Biosynthesis Pathway (404); TNF-alpha/NF-kB Signaling Pathway (198884)

ncbi summary :

This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015]

uniprot summary :

This isozyme may play a role in skeletal mineralization.

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage