antibody

Blood Group Antigen B (CD173) Mouse Monoclonal Antibody

MBS439591

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[HEB-20]

immunohistochemistry, immunocytochemistry

Antibody

Blood Group Antigen B (CD173) Mouse Monoclonal Antibody

[Blood Group Antigen B]

[A transferase; ABO; B transferase; CD173; Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-galactosyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase; Histo-blood group A transferase; Histo-blood group B transferase; NAGAT]

[histo-blood group ABO system transferase; Histo-blood group ABO system transferase; histo-blood group ABO system transferase; ABO blood group (transferase A, alpha 1-3-N-acetylgalactosaminyltransferase; transferase B, alpha 1-3-galactosyltransferase); Fucosylglycoprotein 3-alpha-galactosyltransferase; Fucosylglycoprotein alpha-N-acetylgalactosaminyltransferase; Glycoprotein-fucosylgalactoside alpha-N-acetylgalactosaminyltransferase (EC:2.4.1.40]

[ABO]

[ABO; ABO; GTB; NAGAT; A3GALNT; A3GALT1; A transferase; B transferase]

BGAT_HUMAN

Monoclonal

IgG1, kappa

[HEB-20]

Mouse

Human. others-not known

354

The antibody HEB-20 reacts with human blood group B. The specificity of the antibody HEB-20 was confirmed by comparison of specificity and reactivity to standard reagent using >5.000 samples of blood. The MAb HEB-20 shows specific staining of erythrocytes and vascular epithelium of blood group B controls and no staining in group A controls. This MAb is applicable for tissue staining in tumor patients with blood groups B and AB. Blood group antigens are generally defined as molecules formed by sequential addition of saccharides to the carbohydrate side chains of lipids and proteins detected on erythrocytes and certain epithelial cells. The A, B and H antigens are reported to undergo modulation during malignant cellular transformation. Blood group related antigens represent a group of carbohydrate determinants carried on both glycolipids and glycoproteins. They are usually mucin type, and are detected on erythrocytes, certain epithelial cells, and in secretions of certain individuals. Sixteen genetically and biosynthetically distinct but inter related specificities belong to this group of antigens, including A, B, H, Lewis A, Lewis B, Lewis X, Lewis Y, and precursor type 1 chain antigens.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Agglutination; Immunofluorescence; Immunohistology (Formalin)

Agglutination. Immunofluorescence (0.5-1ug/ml). Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC)

Cellular Localization: Cell Surface. Immunogen: Mixture of erythrocytes of group B and glycoprotein fraction isolated from saliva of secretors with blood group B. Mol. Weight of Antigen: Multiple

Hu-Chromosome Location: 9q34.2. Positive Control: KG1 cells or human colorectal carcinoma tissues. Supplied As: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0ml

Van-k J, Dr-malov- D, Smyslov- O, Nmec M, Viklick- V, Wisniewski K. Detection of blood group A antigen expression in human colon cancer using monoclonal antibodies with different specificities. Neoplasma. 1989;36(4):479-88

58331216

NP_065202.2

NM_020469.2

P16442

Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (82995); Glycosphingolipid Biosynthesis - Lacto And Neolacto Series Pathway (370); Metabolic Pathways (132956)

This gene encodes proteins related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The 'O' blood group is caused by a deletion of guanine-258 near the N-terminus of the protein which results in a frameshift and translation of an almost entirely different protein. Individuals with the A, B, and AB alleles express glycosyltransferase activities that convert the H antigen into the A or B antigen. Other minor alleles have been found for this gene. [provided by RefSeq, Jul 2008]

ABO: This protein is the basis of the ABO blood group system. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of UDP-GalNAc) or to the B antigen (by addition of UDP-Gal), whereas O individuals lack such activity. Belongs to the glycosyltransferase 6 family. Protein type: EC 2.4.1.40; Transferase; EC 2.4.1.37; Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series. Chromosomal Location of Human Ortholog: 9q34.2. Disease: Blood Group, Abo System

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340