CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody | MyBioSource MBS439537 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody

catalog :

MBS439537

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS439537

products type :

Antibody

products full name :

CD11c (Dendritic Cell Marker) Mouse Monoclonal Antibody

products short name :

[CD11c]

products name syn :

[CD11 antigen-like family member C; Complement component 3 receptor 4 subunit; Integrin alpha-X; integrin, alpha X (antigen CD11C (p150), alpha polypeptide); Leu M5 alpha subunit; Leukocyte adhesion glycoprotein p150 95 alpha chain; Myeloid membrane antigen alpha subunit; p150/95]

other names :

[integrin alpha-X isoform 2; Integrin alpha-X; integrin alpha-X; integrin subunit alpha X; CD11 antigen-like family member C; Leu M5; Leukocyte adhesion glycoprotein p150,95 alpha chain; Leukocyte adhesion receptor p150,95; CD_antigen: CD11c]

products gene name :

[ITGAX]

other gene names :

[ITGAX; ITGAX; CD11C; SLEB6; CD11C]

uniprot entry name :

ITAX_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, kappa

host :

Mouse

reactivity :

Human.Others not known.

sequence length :

1163

specificity :

Recognizes a protein of 145kDa, identified as CD11c. CD11c (ITGAX), a member of the leukointegrin family, shares the same beta subunit with other members of the leukocyte adhesion molecule family, which includes CD11a (LFA-1), CD11b (MAC-1) and CD11d (ITGAD), but has a unique alpha chain. CD11c has been shown to play a role in phagocytosis, cell migration, and cytokine production by monocytes/macrophages as well as induction of T-cell proliferation by Langerhans cells. CD11c is expressed prominently on the plasma membranes of monocytes, tissue macrophages, NK cells, and most dendritic cells (DCs). A lower level of expression is also observed on neutrophils as a result of its high level of expression on most DCs. An antibody to CD11c may aid in identification of lesions with histiocytic origin. It may also been used as a marker for hairy cell leukemia in paraffin-embedded tissues.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8°C.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1 ug/million cells). Immunofluorescence (0.5-1ug/mL). Immunohistology (Formalin-fixed)(1-2ug/mL for 30 min at RT). Staining of formalin-fixxed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min. Optimal dilution for specific application should be determined.

other info1 :

Cellular Localization: Cell Surface. Immunogen: Recombinant human ITGAX protein fragment (aa 637-827)

other info2 :

Hu-Chromosome Location: 16p11.2. Positive Control: Human dendritic cells. Human lymph nodes and tonsils.

products references :

Nicolaou, F., et al. 2003. CD11c gene expression in hairy cell leukemia is dependent upon activation of the proto-oncogenes Ras and JunD. Blood 101: 4033-4041

ncbi gi num :

34452173

ncbi acc num :

NP_000878.2

ncbi gb acc num :

NM_000887.4

uniprot acc num :

P20702

ncbi mol weight :

145kDa

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (1269373); Complement And Coagulation Cascades Pathway (83073); Complement And Coagulation Cascades Pathway (484); ECM Proteoglycans Pathway (1270256); Extracellular Matrix Organization Pathway (1270244); Focal Adhesion Pathway (198795); Hemostasis Pathway (1269340); Integrin Cell Surface Interactions Pathway (1270260); Integrin-mediated Cell Adhesion Pathway (198816); Regulation Of Actin Cytoskeleton Pathway (83089)

ncbi summary :

This gene encodes the integrin alpha X chain protein. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This protein combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as inactivated-C3b (iC3b) receptor 4 (CR4). The alpha X beta 2 complex seems to overlap the properties of the alpha M beta 2 integrin in the adherence of neutrophils and monocytes to stimulated endothelium cells, and in the phagocytosis of complement coated particles. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

uniprot summary :

ITGAX: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Belongs to the integrin alpha chain family. Protein type: Membrane protein, integral; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cell surface; membrane; plasma membrane. Molecular Function: protein binding; receptor activity. Biological Process: cell adhesion; extracellular matrix organization and biogenesis; heterotypic cell-cell adhesion; leukocyte migration; organ morphogenesis. Disease: Systemic Lupus Erythematosus

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage