Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody | MyBioSource MBS439477 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody

catalog :

MBS439477

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[SPM595]

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with Glypican-3 Monoclonal Antibody (SPM595)

image 2 :

Formalin-fixed, paraffin-embedded human Melanoma stained with Glypican-3 Monoclonal Antibody (SPM595)

product information

catalog number :

MBS439477

products type :

Antibody

products full name :

Glypican-3 (GPC3) (Hepatocellular Carcinoma Marker) Mouse Monoclonal Antibody

products short name :

[Glypican-3 (GPC3)]

products name syn :

[DGSX; Glypican proteoglycan 3; GPC3; GTR2-2; Heparan sulphate proteoglycan; Intestinal protein OCI-5; MXR7; OCI-5; SDYS; Secreted glypican-3; SGBS1]

other names :

[glypican-3 isoform 1; Glypican-3; glypican-3; glypican 3; GTR2-2; Intestinal protein OCI-5; MXR7]

products gene name :

[GPC3]

other gene names :

[GPC3; GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2; OCI5]

uniprot entry name :

GPC3_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[SPM595]

host :

Mouse

reactivity :

Human. Others not known

sequence length :

603

specificity :

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. Anti-GPC3 has been identified as a useful tumor marker for the diagnosis of hepatocellular carcinoma (HCC), hepatoblastoma, melanoma, testicular germ cell tumors, and Wilms tumor. In patients with HCC, GPC3 is overexpressed in neoplastic liver tissue and elevated in serum, but is undetectable in normal liver, benign liver, and the serum of healthy donors. GPC3 expression is also found to be higher in HCC liver tissue than in cirrhotic liver or liver with focal lesions such as dysplastic nodules and areas of hepatic adenoma (HA) with malignant transformation. In the context of testicular germ cell tumors, GPC3 expression is up regulated in certain histologic subtypes, specifically yolk sac tumors and choriocarcinoma. A high level of GPC3 expression is also found in some types of embryonal tumors, such as Wilms tumor and hepatoblastoma, with a low or undetectable expression in normal adjacent tissue. In patients with thyroid cancer, expression of GPC3 is dramatically enhanced in certain types of cancers: 100% in follicular carcinoma and 70% in papillary carcinoma. Expression of GPC3 in follicular carcinoma is significantly higher than that of follicular adenoma. In contrast, GPC3 is not expressed in anaplastic carcinoma.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody without azide - store at -20°C to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (0.5-1ug/ml). Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: A recombinant fragment containing amino acids 511-580 of human glypican-3

other info2 :

Hu-Chromosome Location: Xq26.2. Positive Control: 293T cells or Hepatocellular carcinoma

products references :

Yan, B., et al. 2011. Expression and clinicopathologic significance of glypican 3 in hepatocellular carcinoma. Ann. Diagn. Pathol. 15: 162-169.

ncbi gi num :

257471006

ncbi acc num :

NP_001158089.1

ncbi gb acc num :

NM_001164617.1

uniprot acc num :

P51654

ncbi mol weight :

67kDa

ncbi pathways :

A Tetrasaccharide Linker Sequence Is Required For GAG Synthesis Pathway (1269981); Chondroitin Sulfate/dermatan Sulfate Metabolism Pathway (1269984); Defective B3GALT6 Causes EDSP2 And SEMDJL1 Pathway (1309217); Defective B3GAT3 Causes JDSSDHD Pathway (1269015); Defective B4GALT7 Causes EDS, Progeroid Type Pathway (1269014); Defective EXT1 Causes Exostoses 1, TRPS2 And CHDS Pathway (1269021); Defective EXT2 Causes Exostoses 2 Pathway (1269022); Disease Pathway (1268854); Diseases Associated With Glycosaminoglycan Metabolism Pathway (1269011); Diseases Of Glycosylation Pathway (1269010)

ncbi summary :

Cell surface heparan sulfate proteoglycans are composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains. Members of the glypican-related integral membrane proteoglycan family (GRIPS) contain a core protein anchored to the cytoplasmic membrane via a glycosyl phosphatidylinositol linkage. These proteins may play a role in the control of cell division and growth regulation. The protein encoded by this gene can bind to and inhibit the dipeptidyl peptidase activity of CD26, and it can induce apoptosis in certain cell types. Deletion mutations in this gene are associated with Simpson-Golabi-Behmel syndrome, also known as Simpson dysmorphia syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2009]

uniprot summary :

Cell surface proteoglycan that bears heparan sulfate. Inhibits the dipeptidyl peptidase activity of DPP4. May be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. May play a role in the modulation of IGF2 interactions with its receptor and thereby modulate its function. May regulate growth and tumor predisposition.

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage