p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody | MyBioSource MBS439425 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody

catalog :

MBS439425

quantity :

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price :

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clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[KP10 + KIP2/880]

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

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image

image 1 :

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with p57 Monoclonal Antibody (KP10+KIP2/880).

product information

catalog number :

MBS439425

products type :

Antibody

products full name :

p57Kip2 (Mitotic Inhibitor/Suppressor Protein) Mouse Monoclonal Antibody

products short name :

[p57Kip2]

products name syn :

[Beckwith Wiedemann syndrome (WBS); BWCR; Cyclin dependent kinase inhibitor 1C (CDKN1C); Cyclin dependent kinase inhibitor p57; KIP2; p57; p57Kip2]

other names :

[cyclin-dependent kinase inhibitor 1C isoform a; Cyclin-dependent kinase inhibitor 1C; cyclin-dependent kinase inhibitor 1C; cyclin dependent kinase inhibitor 1C; Cyclin-dependent kinase inhibitor p57; p57Kip2]

products gene name :

[CDKN1C]

other gene names :

[CDKN1C; CDKN1C; BWS; WBS; p57; BWCR; KIP2; p57Kip2; KIP2]

uniprot entry name :

CDN1C_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[KP10 + KIP2/880]

host :

Mouse

reactivity :

Human, Mouse. Others not known.

sequence length :

316

specificity :

Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes, and is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts and stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts and stromal cells stain. The histological differentiation of complete mole, partial mole, and hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease and choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei and stromal cells are labeled with this antibody. Similar findings apply to PHM and hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities and serve as an internal control.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide store at 2-8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (0.5-1ug/ml). Immunohistology (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Nuclear. Immunogen: Recombinant human p57Kip2 protein

other info2 :

Hu-Chromosome Location: 11p15.5. Positive Control: LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.

products references :

Lee, M.-H., et al. 1995. Cloning of p57, a cyclin-dependent kinase inhibitor with unique domain structure and tissue distribution. Genes Dev. 9: 639-649

ncbi gi num :

4557441

ncbi acc num :

NP_000067.1

ncbi gb acc num :

NM_000076.2

uniprot acc num :

P49918

ncbi mol weight :

57kDa

ncbi pathways :

Cell Cycle Pathway (83054); Cell Cycle Pathway (463); Endochondral Ossification Pathway (198812); G1 To S Cell Cycle Control Pathway (198805)

ncbi summary :

This gene is imprinted, with preferential expression of the maternal allele. The encoded protein is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Oct 2010]

uniprot summary :

p57Kip2: Potent tight-binding inhibitor of several G1 cyclin/CDK complexes (cyclin E-CDK2, cyclin D2-CDK4, and cyclin A-CDK2) and, to lesser extent, of the mitotic cyclin B-CDC2. Negative regulator of cell proliferation. May play a role in maintenance of the non- proliferative state throughout life. Expressed in the heart, brain, lung, skeletal muscle, kidney, pancreas and testis. High levels are seen in the placenta while low levels are seen in the liver. Belongs to the CDI family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Inhibitor; Nuclear receptor co-regulator; Tumor suppressor; Cell cycle regulation. Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: cytoplasm; nucleus. Molecular Function: protein binding. Biological Process: negative regulation of epithelial cell proliferation; negative regulation of kinase activity; negative regulation of phosphorylation; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; positive regulation of transforming growth factor beta receptor signaling pathway. Disease: Beckwith-wiedemann Syndrome; Intrauterine Growth Retardation, Metaphyseal Dysplasia, Adrenal Hypoplasia Congenita, And Genital Anomalies

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