Ep-CAM /CD326 (Rat) (Epithelial Marker) Mouse Monoclonal Antibody | MyBioSource MBS439261 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Ep-CAM /CD326 (Rat) (Epithelial Marker) Mouse Monoclonal Antibody

catalog :

MBS439261

quantity :

0.1 mL (Concentrate)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[Epcam/1158]

reactivity :

human, mouse, rat

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded Rat Lung stained with Ep-CAM Monoclonal Antibody (Epcam/1158).

image 2 :

Formalin-fixed, paraffin-embedded Rat Colon stained with Ep-CAM Monoclonal Antibody (Epcam/1158).

product information

catalog number :

MBS439261

products type :

Antibody

products full name :

Ep-CAM /CD326 (Rat) (Epithelial Marker) Mouse Monoclonal Antibody

products short name :

[Ep-CAM /CD326]

products name syn :

[Adenocarcinoma-associated Antigen; Cell Surface Glycoprotein Trop-1; EGP2; EGP314; EGP40; Epithelial Cell Adhesion Molecule; Epithelial Glycoprotein 314; ESA; KSA; TACD1; TACSTD1; TROP1; Tumor-associated Calcium Signal Transducer 1, ECS-1, Epidermal Surface Antigen 1, ESA1, FLOT2, Flotillin-2, M17S1 (Membrane Component, Chromosome 17, Surface Marker-1), REG-1, Reggie-1, Reggie-2]

other names :

[epithelial cell adhesion molecule; Epithelial cell adhesion molecule; epithelial cell adhesion molecule; epithelial cell adhesion molecule; Epithelial glycoprotein 314; EGP314; Protein D5.7A; Tumor-associated calcium signal transducer 1; CD_antigen: CD326]

products gene name :

[Epcam]

other gene names :

[Epcam; Epcam; Egp314; Tacstd1; Tacstd1; Ep-CAM; EGP314]

uniprot entry name :

EPCAM_RAT

clonality :

Monoclonal

isotype :

IgG1

clone :

[Epcam/1158]

host :

Mouse

reactivity :

Rat, Mouse. Does not react with human

sequence length :

315

specificity :

Epitopes of Epcam/1158 and Epcam/1159 monoclonal antibodies are different. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM). It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas.

form :

Bioreactor Concentrate with 0.05% Azide.

storage stability :

Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistology - Formalin-fixed

app notes :

Flow Cytometry (5-10ul/million cells). Immunofluorescence (1:50-1:100). Western Blot (1:100-1:200). Immunohistology (Formalin-fixed) (1:50-1:100; 30 min at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Cell Surface. Immunogen: Recombinant full-length Rat Epcam protein

other info2 :

Hu-Chromosome Location: 6q12. Positive Control: Recombinant full-length rat Epcam protein

products references :

Litvinov SV et. al. J Cell Biol, 1994, 125:437-46. 2. Tsubura A et. al. J Cut Pathol, 1992, 19:73-9

ncbi gi num :

25742698

ncbi acc num :

NP_612550.1

ncbi gb acc num :

NM_138541.1

uniprot acc num :

O55159

ncbi mol weight :

40-43kDa

ncbi summary :

involved in metastasis formation and tumor progression [RGD, Feb 2006]

uniprot summary :

EPCAM: a single-pass type I membrane protein of the EPCAM family. May act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. Expressed in almost all epithelial cell membranes but not on mesodermal or neural cell membranes. Found on the surface of adenocarcinomas including pancreatic carcinoma. May be a marker of cancer stem cells. Protein type: Membrane protein, integral; Cell surface; Cell cycle regulation; Cell adhesion; Motility/polarity/chemotaxis. Cellular Component: apical plasma membrane; basolateral plasma membrane; cell surface; integral to membrane; integral to plasma membrane; lateral plasma membrane; plasma membrane; tight junction. Molecular Function: protein complex binding. Biological Process: negative regulation of apoptosis; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; stem cell differentiation

size1 :

0.1 mL (Concentrate)

price1 :

190 USD

size2 :

0.5 mL (Concentrate)

price2 :

340

more info or order :

MyBioSource product webpage