antibody

Insulin-like Growth Factor-1 (IGF-1) Mouse Monoclonal Antibody

MBS439149

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

human, mouse, rat

immunocytochemistry, flow cytometry, FACS

Antibody

Insulin-like Growth Factor-1 (IGF-1) Mouse Monoclonal Antibody

[Insulin-like Growth Factor-1 (IGF-1)]

[Insulin-like Growth Factor IA (IGF-IA); Insulin-like Growth Factor IB (IGF-IB); Mechano Growth Factor (MGF); Somatomedin-C]

[insulin-like growth factor I isoform 4 preproprotein; Insulin-like growth factor I; insulin-like growth factor I; insulin like growth factor 1; Mechano growth factor; MGF; Somatomedin-C]

[IGF1]

[IGF1; IGF1; MGF; IGFI; IGF-I; IBP1; IGF-I; MGF]

IGF1_HUMAN

Monoclonal

IgG1, kappa

Mouse

Human, Mouse, Rat, Rabbit

This antibody is specific to Insulin-like Growth Factor (IGF-1) and shows minimal cross-reaction with IGF-11, Proinsulin, MSF, and Insulin. IGF-1 is a polypeptide growth factor with two isoforms that are produced by alternative splicing. Isoform 1 is also known as IGF-IB while isoform 2 is known as IGF-IA. IGF-1 stimulates the proliferation of a wide range of cell types including muscle, bone and cartilage tissue. It functions as an autocrine regulator of growth. Activation of IGF system has emerged as a key factor for tumor progression and resistance to apoptosis in many cancers like those of breast, thyroid and colon.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8°C.

Flow Cytometry (FC/FACS), Immunofluorescence (IF), In-vitro Neutralization of Biological Activity of IGF-1

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). In-vitro Neutralization of Biological Activity of IGF-1 (Order Ab without Azide). Optimal dilution for a specific application should be determined.

Cellular Localization: Cytoplasmic (Secreted). Immunogen: Full-length recombinant human IGF-1 protein

Hu-Chromosome Location: 12q23.2. Positive Control: Pancreas or brain. Breast, Thyroid or Colon Cancers; IGF-1 recombinant protein

Rotwein p, et. al. (1986) J. Biol. Chem. 261: 4828-4832. Sandberg-Nordqvist AC, et. al. (1993) Cancer Res. 53: 2475-2478. Zheng WH, et. al. (2000) J. Neural.Transm. Suppl. 2000: 261-272

11024682

NP_000609.1

NM_000618.4

P05019

AMPK Signaling Pathway (989139); AMPK Signaling Pathway (992181); Adipogenesis Pathway (198832); Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Apoptosis Pathway (198797); Cardiac Progenitor Differentiation Pathway (712094); Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Endochondral Ossification Pathway (198812)

The protein encoded by this gene is similar to insulin in function and structure and is a member of a family of proteins involved in mediating growth and development. The encoded protein is processed from a precursor, bound by a specific receptor, and secreted. Defects in this gene are a cause of insulin-like growth factor I deficiency. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Sep 2015]

IGF1: The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. Defects in IGF1 are the cause of insulin-like growth factor I deficiency (IGF1 deficiency). IGF1 deficiency is an autosomal recessive disorder characterized by growth retardation, sensorineural deafness and mental retardation. Belongs to the insulin family. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted. Chromosomal Location of Human Ortholog: 12q23.2. Cellular Component: extracellular region; extracellular space; insulin-like growth factor binding protein complex; plasma membrane. Molecular Function: hormone activity; insulin receptor binding; insulin-like growth factor receptor binding; integrin binding; protein binding. Biological Process: activation of MAPK activity; activation of protein kinase B; cell activation; cell motility; cell proliferation; cellular protein metabolic process; DNA replication; glycolate metabolic process; insulin-like growth factor receptor signaling pathway; muscle development; muscle hypertrophy; myoblast differentiation; myoblast proliferation; myotube cell development; negative regulation of apoptosis; phosphoinositide 3-kinase cascade; phosphoinositide-mediated signaling; platelet degranulation; positive regulation of activated T cell proliferation; positive regulation of cell migration; positive regulation of cell proliferation; positive regulation of DNA binding; positive regulation of DNA replication; positive regulation of epithelial cell proliferation; positive regulation of fibroblast proliferation; positive regulation of glucose import; positive regulation of glycogen biosynthetic process; positive regulation of glycolysis; positive regulation of insulin-like growth factor receptor signaling pathway; positive regulation of MAPKKK cascade; positive regulation of mitosis; positive regulation of osteoblast differentiation; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein import into nucleus, translocation; positive regulation of protein secretion; positive regulation of Ras protein signal transduction; positive regulation of smooth muscle cell migration; positive regulation of smooth muscle cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; protein kinase B signaling cascade; protein stabilization; proteoglycan biosynthetic process; Ras protein signal transduction; regulation of gene expression; regulation of multicellular organism growth; response to heat; satellite cell compartment self-renewal involved in skeletal muscle regeneration; signal transduction; skeletal development. Disease: Insulin-like Growth Factor I Deficiency

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340