antibody

Thomsen-Friedenreich Antigen /CD176 (Pan Carcinoma Marker) Mouse Monoclonal Antibody

MBS439111

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[A68-B/A11]

immunohistochemistry, immunocytochemistry

Antibody

Thomsen-Friedenreich Antigen /CD176 (Pan Carcinoma Marker) Mouse Monoclonal Antibody

[Thomsen-Friedenreich Antigen /CD176]

[T-F Antigen; TF Antigen; Asialoglycophorin; pan-carcinoma marker; CD176]

Monoclonal

IgM, kappa

[A68-B/A11]

Mouse

Human, Mouse, Rat

Recognizes a disaccharide epitope, Gal1-3GalNAc, of Thomsen-Friedenreich (TF) antigen. It is specific for both anomeric forms of the disaccharide (TF and TF, including related structures on the glycolipid) and shows no cross-reactivity with sialylated glycophorin. The Thomsen-Friedenreich antigen acts as an oncofetal antigen, with low expression in normal adult tissues but increasing to fetal levels of expression in hyperplasia or malignancy. It is considered as a pan-carcinoma marker. This MAb is capable to agglutinate desialylated red blood cells. During metastasis, the ability of malignant cells to form multicellular aggregates via homotypic or heterotypic aggregation and their adhesion to the endothelium are critical. The tumor-associated carbohydrate Thomsen-Friedenreich antigen (Gal-GalNAc) is involved in tumor cell adhesion and tissue invasion. It also causes an immune response, and overexpression of the antigen causes cancer cells to be more sensitive to natural killer cell lysis. The Thomsen-Friedenreich antigen is suppressed in normal healthy cells and represents one of the few chemically well-defined antigens associated with tumor malignancy. The presence of the Thomsen-Friedenreich antigen on the surface of cancer cells may result from a divergence from the normal pathway for O-linked glycosylation in these cells, most likely caused by inappropriate localization of the enzymes involved in synthesis of the disaccharide.

200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. (Also available WITHOUT BSA & azide at 1.0mg/ml.)

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Immunofluorescence (IF), Immunohistology (Formalin-fixed)

Immunofluorescence: 0.5-1ug/ml. Immunohistology (Formalin-fixed): 0.5-1.0ug/ml for 30 minutes at RT. Optimal dilution for a specific application should be determined.

Cellular Localization: Cell Surface. Positive Control: KG1 cells or human colorectal carcinoma tissues. Immunogen: Neuraminidase-treated human red blood cells

Karsten U. 2002. CD176 Workshop panel report. In: Mason D, Simmons D, Buckley C, Schwartz-Albiez R, Hadam M, Saalmuller A, Clark E, Malavasi F, Morrissey JA, Vivier E, et al., editors. Leucocyte Typing VII. Oxford, UK: Oxford University Press. Henderson G. et al. Glycobiology 21(10), 1277-1289 (2011

Multiple

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA at 1mg/ml)

340