antibody

Testosterone Mouse Monoclonal Antibody

MBS439032

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[4E1G2]

ELISA, immunohistochemistry, radioimmunoassay, enzyme immunoassay

Antibody

Testosterone Mouse Monoclonal Antibody

[Testosterone]

[17beta-Hydroxy-3-oxo-4-androstene, 17beta-Hydroxy-4-androsten-3-one, 4-Androsten-17beta-ol-3-one, trans-Testosterone]

Monoclonal

IgG1, kappa

[4E1G2]

Mouse

All species

This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~1010M-1. In competitive binding immunoassay, it reacts with testosterone 100%, 11-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

ELISA (EIA), Radioimmnoassay (RIA), Immunohistochemistry (IHC)

ELISA (For coating, order Ab without BSA); Radioimmunoassay (RIA); Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by coolng at RT for 20 minutes). Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC)

SDS-PAGE

Cellular Localization: Secreted and Cytoplasmic. Immunogen: Testosterone 3 CMO conjugated to BSA

Positive Control: Interstitial or Leydid cells in testis. Specific & Comments: This MAb is highly specific to testosterone. Its affinity constant for testosterone is ~10 10 M -1 . In competitive binding immunoassay, it reacts with testosterone 100%, II-beta-hydroxy testosterone 3.3%, 17-alpha-methyl testosterone <0.1 %, 5-alpha DHT 0.8%, estradiol <0.1 % and progesterone <0.1 %. Testosterone, a steroid hormone from the androgen group, is derived from cholesterol. Testosterone is primarily secreted in the testes, but the ovaries and adrenal glands also produce smaller amounts. It is the principal male sex hormone and is responsible for the maturation of sex organs and the development of male secondary sex characteristics. The hormone is also involved in the growth of muscle mass and increases bone density and maturation. It functions by activating the androgen receptor and converting to estradiol and activation of estrogen receptors. Testosterone has been employed therapeutically to treat many conditions including infertility, lack of libido, osteoporosis, height growth and bone marrow stimulation. Anti-testosterone antibody may also prove useful in identification of testicular tumors arising from interstitial cells.

Fantl, V.E. and Wang, D.Y. 1983. Characterisation of monoclonal antibodies raised against Testosterone. J. Steroid Biochem. 19: 1605-1610

288.42Da

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340