Ksp-Cadherin (Kidney-Specific Cadherin) /CDH16 Mouse Monoclonal Antibody | MyBioSource MBS438943 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Ksp-Cadherin (Kidney-Specific Cadherin) /CDH16 Mouse Monoclonal Antibody

catalog :

MBS438943

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[SPM594]

reactivity :

human, mouse, rat, dog

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with KSP-Cadherin Monoclonal Antibody (SPM594)

product information

catalog number :

MBS438943

products type :

Antibody

products full name :

Ksp-Cadherin (Kidney-Specific Cadherin) /CDH16 Mouse Monoclonal Antibody

products short name :

[Ksp-Cadherin (Kidney-Specific Cadherin) /CDH16]

products name syn :

[Cadherin-16 (CDH16); Kidney-specific cadherin; Ksp-cadherin antibody]

other names :

[cadherin-16 isoform 2; Cadherin-16; cadherin-16; cadherin 16; Kidney-specific cadherin; Ksp-cadherin]

products gene name :

[CDH16]

other gene names :

[CDH16; CDH16; Ksp-cadherin]

uniprot entry name :

CAD16_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[SPM594]

host :

Mouse

reactivity :

Human, Mouse, Rat, Rabbit, Dog. Other not known

specificity :

This MAb recognizes a protein of 130kDa, identified as Ksp-cadherin. Cadherins form a superfamily of related glycoproteins that mediate calcium-dependent cell adhesion and transmit signals from the extracellular matrix to the cytoplasm. Cadherins have been implicated in embryogenesis, tissue morphogenesis, tissue structure maintenance, cell polarization, neoplastic invasiveness and metastasis, and membrane transport. It is suggested that Ksp-cadherin is a marker for terminal differentiation of the basolateral membranes of renal tubular epithelial cells. Within the kidney, Ksp-Cadherin is found exclusively in the basolateral membrane of renal tubular epithelial cells and collecting duct cells, and not in glomeruli, renal interstitial cells, or blood vessels. Ksp-Cadherin has been suggested to distinguish Chromophobe Renal-Cell Carcinoma from Oncocytoma.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2°C to 8°C. Antibody without azide - store at -20°C to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Cell Surface with some cytoplasmic. Immunogen: Recombinant human CDH16 protein

other info2 :

Hu-Chromosome Location: 16q22.1. Positive Control: Normal kidney or renal cell carcinoma.

products references :

Shen, S.S., et al. 2005. Kidney-specific cadherin, a specific marker for the distal portion of the nephron and related renal neoplasms. Mod Pathol. 18: 933-40. Mazal, P.R., et al. 2005. Expression of kidney-specific cadherin distinguishes chromophobe renal cell carcinoma from renal oncocytoma. Hum Pathol. 36: 22-28.

ncbi gi num :

325296969

ncbi acc num :

NP_001191673.1

ncbi gb acc num :

NM_001204744.1

uniprot acc num :

O75309

ncbi mol weight :

130kDa

ncbi summary :

This gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of cadherin genes on chromosome 16q22.1, the gene localizes with superfamily members CDH1, CDH3, CDH5, CDH8 and CDH11. The protein consists of an extracellular domain containing 6 cadherin domains, a transmembrane region and a truncated cytoplasmic domain but lacks the prosequence and tripeptide HAV adhesion recognition sequence typical of most classical cadherins. Expression is exclusively in kidney, where the protein functions as the principal mediator of homotypic cellular recognition, playing a role in the morphogenic direction of tissue development. Alternatively spliced transcript variants encoding distinct isoforms have been identified. [provided by RefSeq, Mar 2011]

uniprot summary :

CDH16: Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 16q22.1. Biological Process: cell adhesion

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage