HLA-DRB (MHC II) Mouse Monoclonal Antibody | MyBioSource MBS438800 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

HLA-DRB (MHC II) Mouse Monoclonal Antibody

catalog :

MBS438800

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[HLA-DRB/1067]

reactivity :

human

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot Anaysis of Ramos Cell Lyste using HLA-DRB Monoclonal Antibody (HLA-DRB/1067).

image 2 :

Formalin-fixed, paraffin-embedded human Histiocytoma stained with HLA-DRB Monoclonal Antibody (HLA-DRB/1067).

image 3 :

Formalin-fixed, paraffin-embedded human Tonsil stained with HLA-DRB Monoclonal Antibody (HLA-DRB/1067).

product information

catalog number :

MBS438800

products type :

Antibody

products full name :

HLA-DRB (MHC II) Mouse Monoclonal Antibody

products short name :

[HLA-DRB]

products name syn :

[DRB1; HLA class II histocompatibility antigen, DR-1 beta chain; HLA-DR-beta 1; HLA-DRB1; human leucocyte antigen DRB1; Leucocyte antigen DR beta 1 chain; lymphocyte antigen DRB1; major histocompatibility complex, class II, DR beta 1; MHC class II HLA-DR beta 1 chain; MHC class II HLA-DR-beta cell surface glycoprotein]

other names :

[major histocompatibility complex, class II, DR beta 1; HLA class II histocompatibility antigen, DRB1-15 beta chain; major histocompatibility complex, class II, DR beta 1; major histocompatibility complex, class II, DR beta 1; DW2.2/DR2.2; MHC class II antigen DRB1*15]

products gene name :

[HLA-DRB1]

other gene names :

[HLA-DRB1; HLA-DRB1; SS1; DRB1; DRw10; HLA-DRB; HLA-DR1B; HLA-DRB2]

uniprot entry name :

2B1F_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, kappa

clone :

[HLA-DRB/1067]

host :

Mouse

reactivity :

Human, Monkey

sequence length :

266

specificity :

This MAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of MAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional AP's. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.1-4ug/ml). Western Blot (0.5-2ug/ml). Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Western Blot (WB)

image4 description :

Western Blot Analysis of Ramos cell and human spleen tissue lysate using HLA-DR MAb (HLA-DRB/I067).

image5 heading :

Immunofluorescence (IF)

image5 description :

Immunofluorescence Analysis of Raji cells labeling HLA-OR with HLA-DR Monoclonal Antibody (HLA-DRB/I067) conjugated with APC (Green). The nuclear counterstain is Reddot (Red)

other info1 :

Cellular Localization: Cell Surface. Immunogen: Activated human peripheral blood mononuclear cells

other info2 :

Hu-Chromosome Location: 6p21.3. Positive Control: Ramos, Daudi or HuT78 cells. Tonsil or lymph node

products references :

Marder RJ, et al. 1985. Lab. Invest. 52:497.2. Norton AJ and Isaacson PG. 1987. Am. J. Pathol. 128:225.3. Hua ZX, et al. 1998. Hum. Pathol. 29(12):1441

ncbi gi num :

155030211

ncbi acc num :

NP_002115.2

ncbi gb acc num :

NM_002124.3

uniprot acc num :

P01911

ncbi mol weight :

28kDa (beta chain)

ncbi pathways :

Adaptive Immune System Pathway (1269171); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Asthma Pathway (83120); Asthma Pathway (532); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

ncbi summary :

HLA-DRB1 belongs to the HLA class II beta chain paralogs. The class II molecule is a heterodimer consisting of an alpha (DRA) and a beta chain (DRB), both anchored in the membrane. It plays a central role in the immune system by presenting peptides derived from extracellular proteins. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa. It is encoded by 6 exons. Exon one encodes the leader peptide; exons 2 and 3 encode the two extracellular domains; exon 4 encodes the transmembrane domain; and exon 5 encodes the cytoplasmic tail. Within the DR molecule the beta chain contains all the polymorphisms specifying the peptide binding specificities. Hundreds of DRB1 alleles have been described and typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. DRB1 is expressed at a level five times higher than its paralogs DRB3, DRB4 and DRB5. DRB1 is present in all individuals. Allelic variants of DRB1 are linked with either none or one of the genes DRB3, DRB4 and DRB5. There are 4 related pseudogenes: DRB2, DRB6, DRB7, DRB8 and DRB9. [provided by RefSeq, Jul 2008]

uniprot summary :

HLA-DRB1 iso2: Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route; where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules; and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments; exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides; autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs; other cells of the gastrointestinal tract; such as epithelial cells; express MHC class II molecules and CD74 and act as APCs; which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen; three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs; CD74 undergoes a sequential degradation by various proteases; including CTSS and CTSL; leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells; the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules; increased acidification produces increased proteolysis and efficient peptide loading. Genetic variation in HLA-DRB1 is a cause of susceptibility to sarcoidosis type 1 (SS1). Sarcoidosis is an idiopathic, systemic, inflammatory disease characterized by the formation of immune granulomas in involved organs. Granulomas predominantly invade the lungs and the lymphatic system, but also skin, liver, spleen, eyes and other organs may be involved. Belongs to the MHC class II family. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cell surface; Golgi membrane; late endosome membrane; lysosomal membrane; membrane; MHC class II protein complex; plasma membrane; trans-Golgi network membrane. Molecular Function: peptide antigen binding. Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; polysaccharide assembly with MHC class II protein complex; T cell costimulation; T cell receptor signaling pathway

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage