Transgelin (SM22-alpha) Mouse Monoclonal Antibody | MyBioSource MBS438730 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Transgelin (SM22-alpha) Mouse Monoclonal Antibody

catalog :

MBS438730

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[SPM606]

reactivity :

human, mouse, cow

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS, immunohistochemistry - frozen section

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-paraffin human Colon Carcinoma stained with Transgelin Monoclonal Antibody (SPM606)

product information

catalog number :

MBS438730

products type :

Antibody

products full name :

Transgelin (SM22-alpha) Mouse Monoclonal Antibody

products short name :

[Transgelin]

products name syn :

[2kDa actin-binding protein; Human 22kDa smooth muscle protein; Protein WS3-10; SMCC; Smooth muscle protein 22-alpha (SM22-alpha); TAGLN; TAGLN1; transgelin; Transgelin variant 2; WS3-10 Human Gene SymbolTAGLNHu Chromosome Location11q23.2]

other names :

[transgelin; Transgelin; transgelin; transgelin; 22 kDa actin-binding protein; Protein WS3-10; Smooth muscle protein 22-alpha; SM22-alpha]

products gene name :

[TAGLN]

other gene names :

[TAGLN; TAGLN; SM22; SMCC; TAGLN1; WS3-10; SM22; WS3-10; SM22-alpha]

uniprot entry name :

TAGL_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[SPM606]

host :

Mouse

reactivity :

Human, Cow, Pig, Rabbit, Mouse

sequence length :

201

specificity :

This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac and skeletal muscle, but is restricted during late fetal development and adulthood to all vascular and visceral smooth muscle cells and low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed) (IHC-F)

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

other info2 :

Hu-Chromosome Location: 11q23.2. Positive Control: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

products references :

Shapland, C., et al. 1993. Purification and properties of transgelin: a transformation and shape change sensitive Actin-gelling protein. J. Cell Biol. 121: 1065-1073

ncbi gi num :

48255907

ncbi acc num :

NP_001001522.1

ncbi gb acc num :

NM_001001522.1

uniprot acc num :

Q01995

ncbi mol weight :

22kDa

ncbi summary :

The protein encoded by this gene is a transformation and shape-change sensitive actin cross-linking/gelling protein found in fibroblasts and smooth muscle. Its expression is down-regulated in many cell lines, and this down-regulation may be an early and sensitive marker for the onset of transformation. A functional role of this protein is unclear. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

uniprot summary :

TAGLN: Actin cross-linking/gelling protein. Involved in calcium interactions and contractile properties of the cell that may contribute to replicative senescence. Belongs to the calponin family. Protein type: Motility/polarity/chemotaxis; Actin-binding; Nuclear receptor co-regulator. Chromosomal Location of Human Ortholog: 11q23.2. Molecular Function: protein binding. Biological Process: epithelial cell differentiation; muscle development

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage