antibody

Double Stranded DNA (dsDNA) (Nuclear Marker) Mouse Monoclonal Antibody

MBS438718

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[AE-2]

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

Antibody

Double Stranded DNA (dsDNA) (Nuclear Marker) Mouse Monoclonal Antibody

[Double Stranded DNA (dsDNA)]

[dsDNA]

Monoclonal

IgG3, kappa

[AE-2]

Mouse

Human

This monoclonal antibody is part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. This MAb recognizes the double stranded DNA in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in human cells. This MAb produces a homogeneous staining pattern in the nucleus of normal and malignant cells. Double Stranded deoxyribonucleic acid (ds DNA) is the genetic material of all cells and many viruses and is a polymer of nucleotides. The monomer consists of phosphorylated 2-deoxyribose N-glycosidically linked to one of four bases, adenine, cytosine, guanine or thymine. These are linked together by 3',5'-phosphodiester bridges. In the Watson-Crick double-helix model, two complementary strands are wound in a right-handed helix and held together by hydrogen bonds between complementary base pairs.

200ug/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

Flow Cytometry (1-2ug/million cells). Immunofluorescence (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires heating tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

Immunohistochemistry (IHC)

Immunohistochemistry (IHC)

Immunofluorescence (IF)

Flow Cytometry (FC/FACS)

Flow Cytometric Analysis of paraformaldehyde-fixed Raji cells with Double Stranded DNA Mouse Monoclonal Antibody (AE-2) followed by goat anti-Mouse IgG-CF488 (Blue) Isotype Control (Red)

SDS-PAGE

SDS-PAGE Analysis Purified Double Stranded DNA Mouse Monoclonal Antibody (AE-2). Confirmation of Purity and Integrity of Antibody.

Cellular Localization: Nuclear. Immunogen: Nuclei of Burkitt's cells

Positive Control: Raji, Jurkat or HeLa cells. Tonsil or Colon.

Epstein, A.L. and Clevenger, C.V., Identification of nuclear antigens in human cells by immunofluorescence, immunoelectron microscopy, and immuno-biochemical methods using monoclonal antibodies. In Progress on nonhistone protein research, Vol. 1, Isaac Bekhor, ed., 1985, CRC Press, Boca Raton, FL, pp 117-137

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340