antibody

HLA-A (MHC I) Mouse Monoclonal Antibody

MBS438658

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[108-2C5]

immunocytochemistry, flow cytometry, FACS

Antibody

HLA-A (MHC I) Mouse Monoclonal Antibody

[HLA-A]

[HLA-A; HLA class 1A; MHC class I HLA-A]

[major histocompatibility complex, class I, B; HLA class I histocompatibility antigen, B-7 alpha chain; major histocompatibility complex, class I, B; major histocompatibility complex, class I, B; MHC class I antigen B*7]

[HLA-A]

[HLA-B; HLA-B; AS; HLAB; Bw-47; Bw-50; SPDA1; B-4901; B-5001; HLA-Cw; HLA-B15; HLA-B39; HLA-B49; HLA-B50; HLA-B55; HLA-B59; HLA-B61; HEL-S-83; HLA-DRB1; HLA-B*45ZJ; HLA-B-3506; HLA-B-3905; HLA-B-5502; HLA-B-5602; HLAB]

1B07_HUMAN

Monoclonal

IgG1, kappa

[108-2C5]

Mouse

Human. Others not known.

362

HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. MHC class I antigens play a role in class I MHC-associated antigen presentation, inhibition of NK cell cytotoxicity, tumor surveillance, and tissue allotransplantation. This MAb is useful for HLA molecular typing of peripheral blood leukocytes as well as a large number of leukemic cell lines. It reacts with an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, -A28, -A29, -A30, -A31 and -Aw33). Its epitope maps between aa65-to-aa80 of the alpha1 domain of the HLA-A. This MAb recognizes an intralocus determinant present on a limited number of HLA-A locus-encoded gene products (HLA-A2, -A3, A28, -A29, -A30, -A31 and -Aw33). Furthermore, by testing its reactivity with HLA-A2 natural variants and mutants, the importance of amino acid residues 79 and/or 80 of the alpha1 domain was demonstrated in the formation of an intralocus HLA-A determinant.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2°C to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS), Immunofluorescence (IF)

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.5-1ug/ml). Optimal dilution for a specific application should be determined

Testing Data (TD)

Flow Cytometry (FC)

Cellular Localization: Cell Surface. Immunogen: Normal human peripheral blood lymphocytes

Hu-Chromosome Location: 6q21.3. Positive Control: Raji, THP-1 and Jurkat cells. Tonsil or lymph node.

Domenech N et al. Hum Immunol 1991, 30(2):140-6 Lozano F et al. Immunogenetics 1989, 30::50-3 Lozano F et al. Tissue Antigens 1990, 35:193-5 Young NT et al. Hum Immunol 1997, 52(1):1-11 Krensky AM et al Transplant Proc 1996, 28(6):3026-8 Hansen JA et al Hematol Oncol Clin North Am 1990, 4(3):507-515

17986001

NP_005505.2

NM_005514.7

P01889

~41kDa

Adaptive Immune System Pathway (1269171); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Antigen Presentation: Folding, Assembly And Peptide Loading Of Class I MHC Pathway (1269194); Antigen Processing And Presentation Pathway (83074); Antigen Processing And Presentation Pathway (485); Antigen Processing-Cross Presentation Pathway (1269195); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533)

HLA-B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta-2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa and its gene contains 8 exons. Exon 1 encodes the leader peptide, exon 2 and 3 encode the alpha1 and alpha2 domains, which both bind the peptide, exon 4 encodes the alpha3 domain, exon 5 encodes the transmembrane region and exons 6 and 7 encode the cytoplasmic tail. Polymorphisms within exon 2 and exon 3 are responsible for the peptide binding specificity of each class one molecule. Typing for these polymorphisms is routinely done for bone marrow and kidney transplantation. Hundreds of HLA-B alleles have been described. [provided by RefSeq, Jul 2008]

HLAB iso7: Involved in the presentation of foreign antigens to the immune system. Belongs to the MHC class I family. Protein type: Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: cell surface; early endosome membrane; endoplasmic reticulum; Golgi apparatus; Golgi membrane; MHC class I protein complex; phagocytic vesicle membrane; plasma membrane. Molecular Function: antigen binding; peptide antigen binding. Biological Process: antigen processing and presentation; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-independent; antigen processing and presentation of peptide antigen via MHC class I; regulation of immune response

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340