CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody | MyBioSource MBS438528 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

catalog :

MBS438528

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[C31.3 + C31.7 + C31.10]

reactivity :

human, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded Colon Carcinoma stained with CD31 Monoclonal Antibody (C31.3+C31.7+C31.10)

image 2 :

Formalin-fixed, paraffin embedded Angiosarcoma stained with CD31 Mouse Monoclonal Antibody (C31.3+C31.7+C31.10).

image 3 :

Western Blot Analysis of human THP-1 cell lysate using CD31 Mouse Monoclonal Antibody (C31.3+C31.7+C31.10).

product information

catalog number :

MBS438528

products type :

Antibody

products full name :

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

products short name :

[CD31 / PECAM-1]

products name syn :

[EndoCAM; PECA1; Platelet Endothelial Cell Adhesion Molecule 1; GPIIA']

other names :

[platelet endothelial cell adhesion molecule; Platelet endothelial cell adhesion molecule; platelet endothelial cell adhesion molecule; platelet and endothelial cell adhesion molecule 1; EndoCAM; GPIIA'; PECA1; CD_antigen: CD31]

products gene name :

[PECAM1]

other gene names :

[PECAM1; PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM; PECAM-1]

uniprot entry name :

PECA1_HUMAN

clonality :

Monoclonal

isotype :

Mouse/IgG1,kappa+ IgG1,kappa+ IgG1,kappa

clone :

[C31.3 + C31.7 + C31.10]

host :

Mouse

reactivity :

Human, Cynomolgus Monkey, Rabbit, Rat

sequence length :

738

specificity :

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules. CD31 is expressed by stem cells of the hematopoietic system and is primarily used to identify and concentrate these cells for experimental studies as well as for bone marrow transplantation. Anti-CD31 has shown to be highly specific and sensitive for vascular endothelial cells. Staining of nonvascular tumors (excluding hematopoietic neoplasms) is rare. CD31 MAb reacts with normal, benign, and malignant endothelial cells which make up blood vessel lining. The level of CD31 expression can help to determine the degree of tumor angiogenesis, and a high level of CD31 expression may imply a rapidly growing tumor and potentially a predictor of tumor recurrence.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.5-1ug/ml). Western Blot (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Testing Data (TD)

image3 heading :

Western Blot (WB)

other info1 :

Cellular Localization: Cell Surface and Cytoplasm of endothelial cells. Immunogen: Recombinant full length human CD31 protein.

other info2 :

Hu-Chromosome Location: 17q23.3. Positive Control: THP-1, Jurkat cells. Tonsil or Angiosarcoma.

products references :

Gratzinger D et. al. Am J Clin Pathol 131:264-278 (2009).

ncbi gi num :

313760624

ncbi acc num :

NP_000433.4

ncbi gb acc num :

NM_000442.4

uniprot acc num :

P16284

ncbi mol weight :

~100kDa (endothelium); ~130kDa (platelets)

ncbi pathways :

Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (1269373); Extracellular Matrix Organization Pathway (1270244); Hemostasis Pathway (1269340); Integrin Cell Surface Interactions Pathway (1270260); Leukocyte Transendothelial Migration Pathway (83083); Leukocyte Transendothelial Migration Pathway (494); Malaria Pathway (152665); Malaria Pathway (152657)

ncbi summary :

The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation. [provided by RefSeq, May 2010]

uniprot summary :

PECAM-1: a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions. Plays a role in modulation of integrin-mediated cell adhesion, transendothelial migration, angiogenesis, apoptosis, cell migration, negative regulation of immune cell signaling, autoimmunity, macrophage phagocytosis, IgE-mediated anaphylaxis and thrombosis. Undergoes alternative splicing, generating multiple isoforms in vascular beds of various tissues. Protein type: Membrane protein, integral; Cell adhesion. Chromosomal Location of Human Ortholog: 17q23.3. Cellular Component: extracellular space; plasma membrane; platelet alpha granule membrane. Molecular Function: protein binding. Biological Process: cell recognition; diapedesis; extracellular matrix organization and biogenesis; leukocyte migration; phagocytosis; platelet degranulation; signal transduction

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage