Androgen Receptor (Marker of Androgen Dependence) Mouse Monoclonal Antibody | MyBioSource MBS438516 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Androgen Receptor (Marker of Androgen Dependence) Mouse Monoclonal Antibody

catalog :

MBS438516

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[AR441 + DHTR/882]

reactivity :

human

application :

immunohistochemistry, immunocytochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Androgen Receptor Monoclonal Antibody (AR441 + DHTR/882).

product information

catalog number :

MBS438516

products type :

Antibody

products full name :

Androgen Receptor (Marker of Androgen Dependence) Mouse Monoclonal Antibody

products short name :

[Androgen Receptor]

products name syn :

[AIS; Dihydrotestosterone receptor (DHTR); HUMARA; HYSP1; Kennedy disease (KD); Nuclear receptor subfamily 3 group C member 4 (NR3C4); SMAX1; Spinal and bulbar muscular atrophy (SBMA); Testicular Feminization (TFM)]

other names :

[androgen receptor isoform 1; Androgen receptor; androgen receptor; androgen receptor; Dihydrotestosterone receptor; Nuclear receptor subfamily 3 group C member 4]

products gene name :

[AR]

other gene names :

[AR; AR; KD; AIS; AR8; TFM; DHTR; SBMA; HYSP1; NR3C4; SMAX1; HUMARA; DHTR; NR3C4]

uniprot entry name :

ANDR_HUMAN

clonality :

Monoclonal

isotype :

IgG

clone :

[AR441 + DHTR/882]

host :

Mouse

reactivity :

Human. Does not react with Mouse. Others not known

sequence length :

920

specificity :

Recognizes a protein of 110kDa, which is identified as androgen receptor (AR). It reacts with full length, and the newly described A form of the receptor. It does not cross react with estrogen, progesterone, or glucocorticoid receptors. The expression of AR is reportedly inversely correlated with histologic grade i.e. well differentiated prostate tumors show higher expression than the poorly differentiated tumors. In prostate cancer, AR has been proposed, as a marker of hormone-responsiveness and thus it may be useful in identifying patients likely to benefit from anti-androgen therapy. Anti-androgen receptor has been useful clinically in differentiating morpheaform basal cell carcinoma (mBCC) from desmoplastic trichoepithelioma (DTE) in the skin. This MAb is superb for staining of formalin/paraffin tissues.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Immunofluorescence (0.5-1ug/ml). Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA buffer, pH 7.5-8.5, for 10-20 mins followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Nuclear. Immunogen: A synthetic peptide, aa 302-318 of human AR (AR441); Recombinant human DHTR protein (DHTR/882)

other info2 :

Hu-Chromosome Location: Xq12. Positive Control: LNCap cells or Prostate carcinoma

products references :

J-nne OA, et al. Androgen receptor and mechanism of androgen action. Ann Med 1993; 25:8

ncbi gi num :

21322252

ncbi acc num :

NP_000035.2

ncbi gb acc num :

NM_000044.3

uniprot acc num :

P10275

ncbi mol weight :

110kDa

ncbi pathways :

Activated PKN1 Stimulates Transcription Of AR (androgen Receptor) Regulated Genes KLK2 And KLK3 Pathway (1269513); Alpha6-Beta4 Integrin Signaling Pathway (198807); Androgen Receptor Signaling Pathway (198806); Coregulation Of Androgen Receptor Activity Pathway (138085); FOXA1 Transcription Factor Network Pathway (137979); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); IL-6 Signaling Pathway (198864); Integrated Breast Cancer Pathway (219801); Integrated Pancreatic Cancer Pathway (711360)

ncbi summary :

The androgen receptor gene is more than 90 kb long and codes for a protein that has 3 major functional domains: the N-terminal domain, DNA-binding domain, and androgen-binding domain. The protein functions as a steroid-hormone activated transcription factor. Upon binding the hormone ligand, the receptor dissociates from accessory proteins, translocates into the nucleus, dimerizes, and then stimulates transcription of androgen responsive genes. This gene contains 2 polymorphic trinucleotide repeat segments that encode polyglutamine and polyglycine tracts in the N-terminal transactivation domain of its protein. Expansion of the polyglutamine tract from the normal 9-34 repeats to the pathogenic 38-62 repeats causes spinal bulbar muscular atrophy (Kennedy disease). Mutations in this gene are also associated with complete androgen insensitivity (CAIS). Two alternatively spliced variants encoding distinct isoforms have been described. [provided by RefSeq, Jul 2016]

uniprot summary :

AR: a nuclear hormone receptor and transcription factor. Regulates gene expression and affects cellular proliferation and differentiation in target tissues. Two splice-variant isoforms have been described. Protein type: Transcription factor; Nuclear receptor; DNA-binding. Chromosomal Location of Human Ortholog: Xq12. Cellular Component: cytoplasm; cytosol; nuclear chromatin; nucleoplasm; nucleus; protein complex. Molecular Function: androgen binding; androgen receptor activity; ATPase binding; beta-catenin binding; chromatin binding; enzyme binding; ligand-dependent nuclear receptor activity; protein binding; receptor binding; transcription factor activity; transcription factor binding. Biological Process: activation of NF-kappaB transcription factor; androgen receptor signaling pathway; cell-cell signaling; intracellular receptor-mediated signaling pathway; negative regulation of cell proliferation; negative regulation of integrin biosynthetic process; positive regulation of cell differentiation; positive regulation of cell proliferation; positive regulation of integrin biosynthetic process; positive regulation of phosphorylation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase III promoter; positive regulation of transcription, DNA-dependent; protein oligomerization; signal transduction; transcription initiation from RNA polymerase II promoter; transcription, DNA-dependent; transport. Disease: Androgen Insensitivity Syndrome; Androgen Insensitivity, Partial; Hypospadias 1, X-linked; Prostate Cancer; Spinal And Bulbar Muscular Atrophy, X-linked 1

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage