Eosinophil Peroxidase | MyBioSource MBS438346 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Eosinophil Peroxidase

catalog :

MBS438346

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

HRP

clone name :

[AHE-1]

reactivity :

human

application :

ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS438346

products type :

Antibody

products full name :

Eosinophil Peroxidase

products short name :

[Eosinophil Peroxidase]

products name syn :

[Eosinophil peroxidase heavy chain; EPER; EPO; EPP; EPX; EPX PEN; PERE]

other names :

[eosinophil peroxidase preproprotein; Eosinophil peroxidase; eosinophil peroxidase; eosinophil peroxidase]

products gene name :

[EPX]

other gene names :

[EPX; EPX; EPO; EPP; EPX-PEN; EPER; EPO; EPP; EPO]

uniprot entry name :

PERE_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[AHE-1]

host :

Mouse

reactivity :

Human

sequence length :

715

specificity :

Peripheral blood granulocytes are classified into neutrophils, basophils and eosinophils according to the staining characteristics of their cytoplasmic granules. Granule proteins are released by physiologic and pharmacologic stimuli and play important roles in both normal and pathological host immune responses. Eosinophil major basic protein and eosinophil peroxidase (EPX) are granule proteins specific to the eosinophil. AHE-1 recognizes human EPX, a granule protein specific to eosinophils. It does not cross-react with eosinophil major basic protein, elastase, cathepsin G, esterase N, thrombin, plasmin, kallikrein, lactoferrin, or transferrin. This MAb stains eosinophils only and does not stain other peripheral blood cells, including platelets, neutrophils, monocytes, lymphocytes or red blood cells. Human EPX gene product can form a tetramer of two light chains and two heavy chains. Other peroxidase family members include myeloperoxidase (MPO), lactoperoxidase (LPO), and thyroid peroxidase (TPO).

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA 0.05% azide. Also available WITHOUT BSA azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. ,Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (IH)

app notes :

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.5-1ug/ml). Optimal dilution for a specific application should be determined.

other info1 :

Localization: Cytoplasmic, granular. Positive Control: MCF-7 cells. Tonsil. Human Chromosomal Location: 17q22

other info2 :

Immunogen: Human eosinophils from a patient with hypereosinophilic syndrome. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide. Supplied as: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

products references :

1. Abbas, A., A.H. Lichtman and J.S. Pober. 1994. Cellular and Molecular Biology, W.B. Saunders Co., Philadelphia, London, Toronto, Tokyo), pp 14-30. 2. Skubitz, K.M., N.P. Christiansen and J.R. Mendiola. 1989. Preparation and characterization of monoclonal antibodies to human neutrophil cathepsin G, lactoferrin, eosinophil peroxidase, and eosinophil major basic protein. J. Leukocyte Biol. 46:109-118. 3. Skubitz, K.M., N.G. Wehner and B.H. Gray. 1988. Preparation and characterization of monoclonal antibody that inhibits human neutrophil elastase activity. J. leukocyte Bio. 44:158-165.

ncbi gi num :

4503595

ncbi acc num :

NP_000493.1

ncbi gb acc num :

NM_000502.4

uniprot acc num :

P11678

ncbi mol weight :

70kDa (55kDa-Heavy Chain; 15kDa-Light Chain)

ncbi pathways :

Asthma Pathway (83120); Asthma Pathway (532)

ncbi summary :

This gene is a member of the peroxidase gene family and is expressed in eosinophils. The encoded precursor protein is processed into covalently attached heavy and light chains to form the mature enzyme, which functions as an oxidant. The enzyme is released at sites of parasitic infection or allergen stimulation to mediate lysis of protozoa or parasitic worms. The gene is found in a cluster of three peroxidase genes at chromosome 17q23. Mutations in this gene result in eosinophil peroxidase deficiency. [provided by RefSeq, Sep 2009]

uniprot summary :

EPX: Mediates tyrosine nitration of secondary granule proteins in mature resting eosinophils. Shows significant inhibitory activity towards Mycobacterium tuberculosis H37Rv by inducing bacterial fragmentation and lysis. Defects in EPX are the cause of eosinophil peroxidase deficiency (EPD). EPD is an autosomal recessive defect where anomalous eosinophils are characterized by nuclear hypersegmentation, hypogranulation, and negative peroxidase and phospholipid staining. Belongs to the peroxidase family. XPO subfamily. Protein type: EC 1.11.1.7; Oxidoreductase. Chromosomal Location of Human Ortholog: 17q23.1. Molecular Function: peroxidase activity; metal ion binding; heme binding. Biological Process: positive regulation of interleukin-4 production; negative regulation of interleukin-10 production; hydrogen peroxide catabolic process; negative regulation of interleukin-5 production; response to oxidative stress; defense response to nematode. Disease: Eosinophil Peroxidase Deficiency

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage