antibody

CD28

MBS438303

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[C28/75]

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

Antibody

CD28

[CD28]

[T-cell-specific surface glycoprotein CD28; Tp44]

[T-cell-specific surface glycoprotein CD28 isoform 2; T-cell-specific surface glycoprotein CD28; T-cell-specific surface glycoprotein CD28; CD28 antigen; CD28 molecule; TP44; CD_antigen: CD28]

[CD28]

[CD28; CD28; Tp44]

CD28_HUMAN

Monoclonal

IgG1, kappa

[C28/75]

Mouse

Human

123

Recognizes a glycoprotein of 44-88kDa, which is identified as CD28. It is the critical T-cell co-stimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its co-stimulation role, CD28 functions in preventing T-cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

supplied as 200ug/ml of AB purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody with azide store at 2 to 8 degree C. Antibody is stable for 24 months.

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies, Immunoprecipitation (IP), Immunohistology (IH)

ELISA (For coating, order Ab without BSA); Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen & Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 1mM EDTA Buffer, pH 8.5-9.5, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.

Localization: Cell surface. Positive Control: Jurkat cells or human lymph nodes and tonsils. Human Chromosomal Location: 2q33.2. Unigene: 591629 (Human)

Immunogen: Recombinant human CD28 protein. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide

1. Marti F, Krause A, Post NH, Lyddane C, Dupont B, Sadelain M, King PD: Negative-feedback regulation of CD28 costimulation by a novel mitogen-activated protein kinase phosphatase, MKP6. J Immunol. 2001;166(1):197-206. 2. Scharschmidt E, Wegener E, Heissmeyer V, Rao A, Krappmann D: Degradation of Bcl10 induced by T-cell activation negatively regulates NF-kappa B signaling. Mol Cell Biol. 2004;24(9):3860-73. 3. Jeong SH, Qiao M, Nascimbeni M, Hu Z, Rehermann B, Murthy K, Liang TJ. Immunization with hepatitis C virus-like particles induces humoral and cellular immune responses in nonhuman primates. J Virol. 2004;78(13):6995-7003.

340545508

NP_001230006.1

NM_001243077.1

P10747

44kDa (monomer); 88kDa (dimer)

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); Cell Adhesion Molecules (CAMs) Pathway (83069)

The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jul 2011]

CD28: a type I plasma membrane protein Involved in T-cell activation, the induction of cell proliferation and cytokine production and promotion of T-cell survival. Binds to B7-1 and -2. Expressed in T cells and plasma cells, but not in less mature B cells. Six splice-variant isoforms have been described. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 2q33. Cellular Component: integral to plasma membrane; plasma membrane; immunological synapse; cytosol; external side of plasma membrane. Molecular Function: identical protein binding; protein binding; SH3/SH2 adaptor activity; protease binding; coreceptor activity. Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of isotype switching to IgG isotypes; positive regulation of viral genome replication; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; positive regulation of translation; nerve growth factor receptor signaling pathway; viral reproduction; positive regulation of mitosis; cytokine biosynthetic process; positive regulation of interleukin-2 biosynthetic process; negative thymic T cell selection; T cell receptor signaling pathway; humoral immune response; regulation of defense response to virus by virus; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of inflammatory response to antigenic stimulus; positive regulation of protein kinase B signaling cascade; cell surface receptor linked signal transduction; T cell costimulation; innate immune response; positive regulation of alpha-beta T cell proliferation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of T cell proliferation; regulatory T cell differentiation

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340