antibody

Fibronectin

MBS438250

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monoclonal

mouse

nonconjugated

[2755-8]

human, mouse, rat

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

Antibody

Fibronectin

[Fibronectin]

[Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3]

[fibronectin isoform 3 preproprotein; Fibronectin; fibronectin; cold-insoluble globulin; migration-stimulating factor; fibronectin 1; Cold-insoluble globulin; CIG]

[FN1]

[FN1; FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; FN; FN; CIG]

FINC_HUMAN

Monoclonal

IgG1, kappa

[2755-8]

Mouse

Human, Pig, Mouse, Rat. Others not known

2355

Fibronectin is an extracellular matrix glycoprotein present on most cell surfaces, in extracellular fluids and in plasma. A high molecular weight heterodimeric protein, it was originally discovered as a protein missing from the surfaces of virus-transformed cells, and it has been shown to be involved in various functions including cell adhesion, cell motility and wound healing. Alternative splicing and glycosylation give rise to several different forms of Fibronectin, some of which exhibit restricted tissue distribution or association with malignancies. It has been shown that Myofibroblast phenotype formation correlates with the occurrence of glycosylated Fibronectin and Fibronectin splice variants in Dupuytren's disease.

200 ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA and azide at 1.0 mg/ml

Antibody with azide- Store at 2 to 8 degree C. Antibody is stable for 24 months.

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (IH)

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.5-1ug/ml). Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

Immunohistology

Localization: Connective tissue matrix. Positive Control: SW156 cells or Kidney. Human Chromosomal Location: 2q35

Immunogen: T-cell lymphoma biopsy

1. Epstein AL, et. al. Cancer Research, 1995, 55(12):2673-80.

16933542

NP_002017.1

NM_002026.2

P02751

220kDa (monomer); 440kDa (dimer)

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Surface Interactions At The Vascular Wall Pathway (106062); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Fibronectin Matrix Formation Pathway (771601)

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis; Cell adhesion. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: extracellular matrix; extracellular space; fibrinogen complex; apical plasma membrane; extracellular region; ER-Golgi intermediate compartment; basal lamina. Molecular Function: integrin binding; heparin binding; collagen binding; protein binding; protease activator activity; protease binding. Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; response to wounding; acute-phase response; cell-substrate junction assembly; calcium-independent cell-matrix adhesion; peptide cross-linking; angiogenesis; blood coagulation; cell adhesion; leukocyte migration. Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency

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