MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody | MyBioSource MBS438243 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody

catalog :

MBS438243

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[45M1]

reactivity :

human, mouse, rat, cow

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Stomach stained with MUC5AC Monoclonal Antibody (45M1).

image 2 :

Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

image 3 :

Formalin-fixed, paraffin-embedded Rat Stomach stained with MUC5AC Monoclonal Antibody (45M1).

product information

catalog number :

MBS438243

products type :

Antibody

products full name :

MUC5AC (Mucin 5AC / Gastric Mucin) Mouse Monoclonal Antibody

products short name :

[MUC5AC (Mucin 5AC / Gastric Mucin)]

products name syn :

[Apomucin Major Airway Glycoprotein, Mucin 5 subtype AC tracheobronchial, Mucin 5 Subtypes A And C, Mucin 5AC oligomeric mucus/gel forming, Tracheobronchial Mucin (TBM)]

other names :

[mucin-5AC; Mucin-5AC; mucin-5AC; mucin 5AC, oligomeric mucus/gel-forming; Gastric mucin]

products gene name :

[MUC5AC]

other gene names :

[MUC5AC; MUC5AC; TBM; leB; MUC5; mucin; TBM]

uniprot entry name :

MUC5A_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[45M1]

host :

Mouse

reactivity :

Human, Monkey, Rabbit, Cat, Mouse, Rat, Pig, Hedgehog, Chicken. Does not react with Cow. Others-not-known

sequence length :

5654

specificity :

This MAb recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC). Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Store at 2 to 8 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient

other info2 :

Hu-Chromosome Location: 11p15.5. Positive Control: Stomach

products references :

Cancer Res.46: 3983-3989 (1986). Biochem. J. 254: 185-193 (1988). Int. J. Cancer 47: 304-310 (1991).

ncbi gi num :

748983076

ncbi acc num :

NP_001291288.1

ncbi gb acc num :

NM_001304359.1

uniprot acc num :

P98088

ncbi mol weight :

>1,000kDa

ncbi pathways :

Defective C1GALT1C1 Causes Tn Polyagglutination Syndrome (TNPS) Pathway (1383050); Defective GALNT12 Causes Colorectal Cancer 1 (CRCS1) Pathway (1383052); Defective GALNT3 Causes Familial Hyperphosphatemic Tumoral Calcinosis (HFTC) Pathway (1383051); Disease Pathway (1268854); Diseases Associated With O-glycosylation Of Proteins Pathway (1383049); Diseases Of Glycosylation Pathway (1269010); Metabolism Of Proteins Pathway (1268677); O-linked Glycosylation Pathway (1268734); O-linked Glycosylation Of Mucins Pathway (1268736); Post-translational Protein Modification Pathway (1268701)

uniprot summary :

MUC5AC iso1: . Chromosomal Location of Human Ortholog: 11p15.5. Cellular Component: cytoplasm; extracellular space; fibril; Golgi lumen. Molecular Function: extracellular matrix structural constituent. Biological Process: fibril organization and biogenesis; O-glycan processing; phosphoinositide-mediated signaling

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage