Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody | MyBioSource MBS438228 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

catalog :

MBS438228

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[VM452]

reactivity :

human, mouse, rat, dog, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot Analysis Raji Cell Lysate, Vimentin Mouse Monoclonal Antibody (VM452)

image 2 :

Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452)

image 3 :

Western Blot Analysis of human A375 cell lysate using Vimentin Mouse Monoclonal Antibody (VM452)

product information

catalog number :

MBS438228

products type :

Antibody

products full name :

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

products short name :

[Vimentin]

products name syn :

[VIM]

other names :

[vimentin; Vimentin; vimentin; vimentin]

products gene name :

[VIM]

other gene names :

[VIM; VIM; HEL113; CTRCT30]

uniprot entry name :

VIME_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[VM452]

host :

Mouse

reactivity :

Human, Cow, Dog, Cat, Pig, Goat, Chicken. Does not react with Mouse and Rat

sequence length :

466

specificity :

This MAb reacts with a 58kDa protein identified as vimentin. It shows no cross-reaction with other closely related intermediate filament proteins (IFP's) such as desmin, keratin, neurofilament, and glial fibrillary acid protein. Anti-vimentin alone is of limited value as a diagnostic tool; however, when used in panels with other antibodies, it is useful for the sub-classification of a given tumor. Expression of vimentin, when used in conjunction with anti-keratin, is helpful when distinguishing melanomas from undifferentiated carcinomas and large cell lymphomas. All melanomas and Schwannomas react strongly with anti-vimentin. It labels a variety of mesenchymal cells, including melanocytes, lymphocytes, endothelial cells, and fibroblasts. Non-reactivity of anti-vimentin is often considered more useful than its positive reactivity, since there are a few tumors that do not contain vimentin, e.g. hepatoma and seminoma. Anti-vimentin is also useful as a tissue process control reagent.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Western Blot (0.25-0.5ug/ml). Immunohistochemistry (Formalin-fixed) (0.25-0.5ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Western Blot (WB)

image4 heading :

SDS-PAGE

image4 description :

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). Confirmation of Integrity and Purity of Antibody.

image5 heading :

Flow Cytometry (FC)

image5 description :

Flow Cytometry of human Vimentin on Jurkat Cells, Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: Recombinant full-length human vimentin protein

other info2 :

Hu-Chromosome Location: 10p12.33. Positive Control: Raji, Jurkat or HeLa cells. Sarcomas or Melanomas.

products references :

Osborn M et. al. European Journal of Cell Biology. 1984; 34:137-143

ncbi gi num :

62414289

ncbi acc num :

NP_003371.2

ncbi gb acc num :

NM_003380.3

uniprot acc num :

P08670

ncbi mol weight :

57-60kDa

ncbi pathways :

Alpha6-Beta4 Integrin Signaling Pathway (198807); Apoptosis Pathway (1270262); Apoptotic Cleavage Of Cellular Proteins Pathway (1270288); Apoptotic Execution Phase Pathway (1270287); Aurora B Signaling Pathway (138080); Caspase Cascade In Apoptosis Pathway (137974); Caspase-mediated Cleavage Of Cytoskeletal Proteins Pathway (1270289); Epstein-Barr Virus Infection Pathway (585562); Epstein-Barr Virus Infection Pathway (587115); MicroRNAs In Cancer Pathway (852705)

ncbi summary :

This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract.[provided by RefSeq, Jun 2009]

uniprot summary :

Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally.

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage