antibody

Fibronectin

MBS438210

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[TV-1]

human, mouse, rat

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

Antibody

Fibronectin

[Fibronectin]

[Cold insoluble globulin (CIG); FINC; FN1; FNZ; GFND; GFND2; LETS; Migration stimulating factor (MSF); Ugl-Y3]

[fibronectin isoform 3 preproprotein; Fibronectin; fibronectin; cold-insoluble globulin; migration-stimulating factor; fibronectin 1; Cold-insoluble globulin; CIG]

[FN1]

[FN1; FN1; FN; CIG; FNZ; MSF; ED-B; FINC; GFND; LETS; GFND2; FN; FN; CIG]

FINC_HUMAN

Monoclonal

IgG1, kappa

[TV-1]

Mouse

Human, Pig, Mouse and Rat. Others-not known.

2355

Fibronectin is a soluble dimeric glycoprotein of 440kDa, which is present in cells, extracellular matrix, and blood. This MAb reacts with the cellular as well as plasma form of fibronectin. Reportedly, after iv administration, this MAb localizes to tumor vessels where it binds to the underlying basement. Epitope recognized by this antibody is not accessible in normal tissues to the circulating MAb indicating that it can be used to specifically target tumor vessels in vivo. TV-1 is reportedly useful for delivering vasoactive agents to tumors to induce increased vascular permeability or blood flow prior to treatment with chemotherapeutic drugs or MAbs.

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)

Flow Cytometry (0.5-1ug/million cells in 0.1ml) Immunofluorescence (0.5-1ug/ml) Immunohistology (Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

Immunohistochemistry

Cellular Localization: Connective tissue matrix. Positive Control: SW156 cells or Kidney. Human Chromosomal Location: 2q35

Immunogen: T-cell lymphoma biopsy. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide. Supplied As: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

1. Epstein AL, et. al. Cancer Research, 1995, 55(12):2673-80.

16933542

NP_002017.1

NM_002026.2

P02751

220kDa (monomer); 440kDa (dimer)

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Angiopoietin Receptor Tie2-mediated Signaling Pathway (137917); Bacterial Invasion Of Epithelial Cells Pathway (149807); Bacterial Invasion Of Epithelial Cells Pathway (148661); Cell Surface Interactions At The Vascular Wall Pathway (106062); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); Extracellular Matrix Organization Pathway (576262); Fibronectin Matrix Formation Pathway (771601)

This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

FN1: Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Defects in FN1 are the cause of glomerulopathy with fibronectin deposits type 2 (GFND2); also known as familial glomerular nephritis with fibronectin deposits or fibronectin glomerulopathy. GFND is a genetically heterogeneous autosomal dominant disorder characterized clinically by proteinuria, microscopic hematuria, and hypertension that leads to end-stage renal failure in the second to fifth decade of life. 15 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted; Cell adhesion; Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 2q34. Cellular Component: extracellular matrix; extracellular space; apical plasma membrane; fibrinogen complex; ER-Golgi intermediate compartment; extracellular region; basal lamina. Molecular Function: collagen binding; heparin binding; integrin binding; protein binding; protease activator activity; protease binding. Biological Process: integrin activation; platelet activation; extracellular matrix organization and biogenesis; positive regulation of axon extension; extracellular matrix disassembly; regulation of cell shape; platelet degranulation; calcium-independent cell-matrix adhesion; cell-substrate junction assembly; acute-phase response; response to wounding; peptide cross-linking; angiogenesis; cell adhesion; blood coagulation; leukocyte migration. Disease: Glomerulopathy With Fibronectin Deposits 2; Plasma Fibronectin Deficiency

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340