Tyrosinase-Related Protein-1 (TRP-1) (Melanoma Marker) | MyBioSource MBS438198 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Tyrosinase-Related Protein-1 (TRP-1) (Melanoma Marker)

catalog :

MBS438198

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[TA99]

reactivity :

human, mouse

application :

western blot, ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS438198

products type :

Antibody

products full name :

Tyrosinase-Related Protein-1 (TRP-1) (Melanoma Marker)

products short name :

[Tyrosinase-Related Protein-1 (TRP-1)]

products name syn :

[5; 6 dihydroxyindole 2 carboxylic acid oxidase; 6-dihydroxyindole-2-carboxylic acid oxidase; Associated with iris pigmentation; CAS2; Catalase B (CATB); DHICA oxidase; Glycoprotein75 (GP75); Melanoma antigen gp75; Tyrosinase-related protein 1 (TYRP1); TYRRP]

other names :

[5,6-dihydroxyindole-2-carboxylic acid oxidase; 5,6-dihydroxyindole-2-carboxylic acid oxidase; 5,6-dihydroxyindole-2-carboxylic acid oxidase; catalase B; DHICA oxidase; glycoprotein 75; melanoma antigen gp75; tyrosinase-related protein 1; Catalase B; Glycoprotein 75; Melanoma antigen gp75; Tyrosinase-related protein 1; TRP; TRP-1; TRP1]

products gene name :

[TYRP1]

other gene names :

[TYRP1; TYRP1; TRP; CAS2; CATB; GP75; OCA3; TRP1; TYRP; b-PROTEIN; CAS2; TYRP; TYRRP; DHICA oxidase; TRP; TRP-1; TRP1]

uniprot entry name :

TYRP1_HUMAN

clonality :

Monoclonal

isotype :

IgG2a, kappa

clone :

[TA99]

host :

Mouse

reactivity :

Human and MOuse. Others not known.

sequence length :

537

specificity :

It reacts with a 75kDa melanocyte-specific gene product, identified as Tyrosinase-related protein-1 (TRP-1). It is involved in melanin synthesis. TRP1 is present on the melanosomal membranes of melanoma, normal melanocytes and nevi. Recent evidence suggests that TRP-1 is involved in maintaining stability of tyrosinase protein and modulating its catalytic activity. TRP-1 is also involved in maintenance of melanosome ultrastructure and affects melanocyte proliferation and cell death.

form :

Supplied as 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide.

storage stability :

Store at 2 to 8 degree C. Antibody is stable for 24 months.

tested application :

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP), Immunohistology (IH)

app notes :

Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (1-2ug/ml); Immunohistology (Frozen only) (0.5-1ug/ml for 30 minutes at RT). Optimal dilution for a specific application should be determined.

other info1 :

Localization: Cytoplasmic. Positive Control: SK-MEL-23, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma. Human Chromosomal Location: 9p23. Unigene: 270279 (Human); 30438 (Mouse)

other info2 :

Immunogen: SK-MEL-23 cells. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide

products references :

1. FASEB J. 2010;24(5):1616-29. 2. Clin Cancer Res. 2007;13:566-75.

ncbi gi num :

4507757

ncbi acc num :

NP_000541.1

ncbi gb acc num :

NM_000550.2

uniprot acc num :

P17643

ncbi mol weight :

75kDa

ncbi pathways :

Direct P53 Effectors Pathway (137939); Melanogenesis Pathway (83092); Melanogenesis Pathway (504); Metabolic Pathways (132956); Tyrosine Metabolism Pathway (82959); Tyrosine Metabolism Pathway (325); Ethanol Degradation IV Pathway (142308); Superoxide Radicals Degradation Pathway (142440)

ncbi summary :

This gene encodes a melanosomal enzyme that belongs to the tyrosinase family and plays an important role in the melanin biosynthetic pathway. Defects in this gene are the cause of rufous oculocutaneous albinism and oculocutaneous albinism type III. [provided by RefSeq, Mar 2009]

uniprot summary :

TYRP1: Oxidation of 5,6-dihydroxyindole-2-carboxylic acid (DHICA) into indole-5,6-quinone-2-carboxylic acid. May regulate or influence the type of melanin synthesized. Defects in TYRP1 are the cause of albinism oculocutaneous type 3 (OCA3); also known as Rufous oculocutaneous albinism. An autosomal recessive disorder in which the biosynthesis of melanin pigment is reduced in skin, hair, and eyes. Tyrosinase activity is normal and patients have only moderate reduction of pigment. The eyes present red reflex on transillumination of the iris, dilution of color of iris, nystagmus and strabismus. Darker-skinned individuals have bright copper-red coloration of the skin and hair. Belongs to the tyrosinase family. Protein type: EC 1.14.18.-; Membrane protein, integral; Oxidoreductase; Amino Acid Metabolism - tyrosine. Chromosomal Location of Human Ortholog: 9p23. Cellular Component: melanosome membrane; integral to membrane; melanosome; endosome membrane. Molecular Function: protein binding; protein homodimerization activity; copper ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, another compound as one donor, and incorporation of one atom of oxygen; protein heterodimerization activity. Biological Process: melanin biosynthetic process; melanosome organization and biogenesis; melanocyte differentiation; acetoacetic acid metabolic process. Disease: Albinism, Oculocutaneous, Type Iii; Skin/hair/eye Pigmentation, Variation In, 11

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage