Cytokeratin 14 (KRT14) (Squamous Cell Marker) Mouse Monoclonal Antibody | MyBioSource MBS438196 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Cytokeratin 14 (KRT14) (Squamous Cell Marker) Mouse Monoclonal Antibody

catalog :

MBS438196

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[SPM263]

reactivity :

human, mouse, rat

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).

image 2 :

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Cytokeratin 14 Monoclonal Antibody (SPM263).

image 3 :

SDS-PAGE Analysis Purified Cytokeratin 14 MAb (SPM263). Confirmation of Integrity and Purity of Antibody.

product information

catalog number :

MBS438196

products type :

Antibody

products full name :

Cytokeratin 14 (KRT14) (Squamous Cell Marker) Mouse Monoclonal Antibody

products short name :

[Cytokeratin 14 (KRT14)]

products name syn :

[CK-14; Dowling Meara; ebs3; ebs4; Epidermolysis Bullosa Simplex; k14; Keratin 14; Keratin Type I Cytoskeletal 14; Koebner; krt14; NFJ]

other names :

[keratin, type I cytoskeletal 14; Keratin, type I cytoskeletal 14; keratin, type I cytoskeletal 14; keratin 14; Cytokeratin-14; CK-14; Keratin-14; K14]

products gene name :

[KRT14]

other gene names :

[KRT14; KRT14; K14; NFJ; CK14; EBS3; EBS4; CK-14; K14]

uniprot entry name :

K1C14_HUMAN

clonality :

Monoclonal

isotype :

IgG3

clone :

[SPM263]

host :

Mouse

reactivity :

Human, Mouse, Rat

sequence length :

472

specificity :

Cytokeratin 14 (CK14) belongs to the type I (or A or acidic) subfamily of low molecular weight keratins and exists in combination with keratin 5 (type II or B or basic). CK14 is found in basal cells of squamous epithelia, some glandular epithelia, myoepithelium, and mesothelial cells. Anti-CK14 is useful in differentiating squamous cell carcinomas from poorly differentiated epithelial tumors. Anti-CK14 is one of the specific basal markers for distinguishing between basal and non-basal subtypes of breast carcinomas. Anti-CK14 is also a good marker for differentiation of intraductal from invasive salivary duct carcinoma by the positive staining of basal cells surrounding the in-situ neoplasm as well as for differentiation of benign prostate from prostate carcinoma. Furthermore, this antibody has been useful in separating oncocytic tumors of the kidney from its renal mimics, and in identifying metaplastic carcinomas of the breast.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 min at RT). (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

SDS-PAGE

image4 heading :

Immunofluorescence (IF)

image4 description :

Immunofluorescence Analysis of A549 Cells labeling KRT14 with Cytokeratin 14 MAb (SPM263) followed by Goat anti-mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red).

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: A synthetic peptide of 15 amino acid from the C-terminus of human keratin 14.

other info2 :

Hu-Chromosome Location: 17q21.2. Positive Control: A431 cells. Skin or Squamous cell carcinoma

products references :

Perkins W et. al. J Cutaneous Pathol, 1992, 19(6):476-82. Kasper M. Histochemistry, 1991, 95(6):613-20. Leigh I M et. al. (1988) Dermatology, 91:415

ncbi gi num :

15431310

ncbi acc num :

NP_000517.2

ncbi gb acc num :

NM_000526.4

uniprot acc num :

P02533

ncbi mol weight :

50kDa

ncbi pathways :

Corticotropin-releasing Hormone Pathway (920957); Glucocorticoid Receptor Regulatory Network Pathway (138014)

ncbi summary :

This gene encodes a member of the keratin family, the most diverse group of intermediate filaments. This gene product, a type I keratin, is usually found as a heterotetramer with two keratin 5 molecules, a type II keratin. Together they form the cytoskeleton of epithelial cells. Mutations in the genes for these keratins are associated with epidermolysis bullosa simplex. At least one pseudogene has been identified at 17p12-p11. [provided by RefSeq, Jul 2008]

uniprot summary :

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21.2. Cellular Component: cytoplasm; cytosol; intermediate filament; keratin filament; nucleus. Molecular Function: protein binding; structural constituent of cytoskeleton. Biological Process: aging; epidermis development; hair cycle; hemidesmosome assembly; intermediate filament bundle assembly. Disease: Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex, Dowling-meara Type; Epidermolysis Bullosa Simplex, Generalized; Epidermolysis Bullosa Simplex, Localized; Naegeli Syndrome

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage