GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody | MyBioSource MBS438192 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody

catalog :

MBS438192

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[SPM507]

reactivity :

human, mouse, rat, chicken, cow

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Cerebellum stained with GFAP Monoclonal Antibody (SPM507).

image 2 :

Western Blot Analysis of Human Brain Tissue Lysate using GFAP Mab (SPM507).

image 3 :

SDS-PAGE Analysis . Purified EGFR Mouse Monoclonal Antibody (GFR/2596). Confirmation of Integrity and Purity of Antibody

product information

catalog number :

MBS438192

products type :

Antibody

products full name :

GFAP (Astrocyte & Neural Stem Cell Marker) Mouse Monoclonal Antibody

products short name :

[GFAP]

products name syn :

[Astrocyte or Intermediate Filament Protein, Glial Fibrillary Acidic Protein]

other names :

[glial fibrillary acidic protein isoform 2; Glial fibrillary acidic protein; glial fibrillary acidic protein; glial fibrillary acidic protein]

products gene name :

[GFAP]

other gene names :

[GFAP; GFAP; ALXDRD; GFAP]

uniprot entry name :

GFAP_HUMAN

clonality :

Monoclonal

isotype :

IgG1

clone :

[SPM507]

host :

Mouse

reactivity :

Human, Mouse, Rat, Cow, Pig, Rabbit, Chicken. Others not known.

sequence length :

431

specificity :

This MAb recognizes a protein of ~50kDa which is identified as Glial Fibrillary Acidic Protein (GFAP). It shows no cross-reaction with other intermediate filament proteins. GFAP is specifically found in astroglia. GFAP is a very popular marker for localizing benign astrocyte and neoplastic cells of glial origin in the central nervous system. Antibody to GFAP is useful in differentiating primary gliomas from metastatic lesions in the brain and for documenting astrocytic differentiation in tumors outside the CNS.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (1-2ug/ml). Western Blotting (0.5-1ug/ml). Immunohistology (Formalin-fixed) (0.5-1 ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Western Blot (WB)

image3 heading :

SDS-PAGE

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: GFAP isolated from pig spinal cord

other info2 :

Hu-Chromosome Location: 17q21.31. Positive Control: Brain or Astrocytoma

products references :

Herpers MJ et. Al. 1986, Acta Neuropathol, 70:333-339. Van Muijen GN et. al. 1987, Lab Invest, 57:359-369. Debus E, et. al. 1983, Differentiation, 25(2):193-203

ncbi gi num :

196115290

ncbi acc num :

NP_001124491.1

ncbi gb acc num :

NM_001131019.2

uniprot acc num :

P14136

ncbi mol weight :

~50kDa

ncbi pathways :

Jak-STAT Signaling Pathway (83077); Jak-STAT Signaling Pathway (488); Neural Crest Differentiation Pathway (672460); Nuclear Signaling By ERBB4 Pathway (1269499); Signal Transduction Pathway (1269379); Signaling By ERBB4 Pathway (1269496); Spinal Cord Injury Pathway (739007)

ncbi summary :

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Oct 2008]

uniprot summary :

GFAP: a class-III intermediate filament protein. A cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. An additional transcript variant isoform has been described, but its full length sequence has not been determined. Protein type: Cytoskeletal. Chromosomal Location of Human Ortholog: 17q21. Cellular Component: cytoplasm; cytosol; intermediate filament cytoskeleton; lysosome. Molecular Function: protein binding. Biological Process: regulation of protein complex assembly. Disease: Alexander Disease

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage