Tyrosinase (Melanoma Marker) Mouse Monoclonal Antibody | MyBioSource MBS438190 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Tyrosinase (Melanoma Marker) Mouse Monoclonal Antibody

catalog :

MBS438190

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[T311]

reactivity :

human

application :

immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-paraffin melanoma (10x) stained with Tyrosinase MAB(T311).

image 2 :

Formalin-paraffin melanoma( 20x) stained wtih Tyrosinase MAb(T311).

product information

catalog number :

MBS438190

products type :

Antibody

products full name :

Tyrosinase (Melanoma Marker) Mouse Monoclonal Antibody

products short name :

[Tyrosinase]

products name syn :

[ATN, CMM8, LB24-AB, Monophenol monooxygenase, OCA1, OCA1A, Oculocutaneous albinism IA, SHEP3, SK29-AB, Tumor rejection antigen AB, TYR]

other names :

[tyrosinase; Tyrosinase; LB24-AB; Monophenol monooxygenase; SK29-AB; Tumor rejection antigen AB]

products gene name :

[TYR]

other gene names :

[TYR]

uniprot entry name :

TYRO_HUMAN

clonality :

Monoclonal

isotype :

IgG2a, kappa

clone :

[T311]

host :

Mouse

reactivity :

Human.Others not known.

sequence length :

529

specificity :

Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1 and tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa), and the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes and melanomas.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide- store at 2-8°C

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Immunohistology(Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, or 10mM . Citrate buffer, pH6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry IHC

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: Recombinant tyrosinase protein

other info2 :

Hu-Chromosome Location: 11q14.3. Positive Control: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.

products references :

Chen Y-T, et. al. Proc. Natl. Acad. Sci. USA, 1995, 92:8125-8129

ncbi gi num :

4507753

ncbi gb acc num :

NM_000372.4

uniprot acc num :

P14679

ncbi mol weight :

70-80kDa

ncbi pathways :

(S)-reticuline Biosynthesis Pathway (547499); (S)-reticuline Biosynthesis II Pathway (138845); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (413357); Catecholamine Biosynthesis, Tyrosine = Dopamine = Noradrenaline = Adrenaline Pathway (468235); Dopamine Metabolism Pathway (920995); L-dopachrome Biosynthesis Pathway (545322); L-dopachrome Biosynthesis Pathway (545496); Melanin Biosynthesis Pathway (1270185); Melanogenesis Pathway (83092); Melanogenesis Pathway (504)

uniprot summary :

TYR: This is a copper-containing oxidase that functions in the formation of pigments such as melanins and other polyphenolic compounds. Catalyzes the rate-limiting conversions of tyrosine to DOPA, DOPA to DOPA-quinone and possibly 5,6-dihydroxyindole to indole-5,6 quinone. Increased expression after UVB irradiation. Belongs to the tyrosinase family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Oxidoreductase; Cofactor and Vitamin Metabolism - riboflavin; Amino Acid Metabolism - tyrosine; EC 1.14.18.1. Chromosomal Location of Human Ortholog: 11q14.3. Cellular Component: cytoplasm; Golgi-associated vesicle; lysosome; melanosome; melanosome membrane; perinuclear region of cytoplasm. Molecular Function: copper ion binding; monooxygenase activity; monophenol monooxygenase activity; protein binding; protein heterodimerization activity; protein homodimerization activity. Biological Process: eye pigment biosynthetic process; melanin biosynthetic process; melanin biosynthetic process from tyrosine; visual perception. Disease: Albinism, Ocular, With Sensorineural Deafness; Albinism, Oculocutaneous, Type Ia; Albinism, Oculocutaneous, Type Ib; Skin/hair/eye Pigmentation, Variation In, 3

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage