PAX7 (Rhabdomyosarcoma Marker) Mouse Monoclonal Antibody | MyBioSource MBS438177 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

PAX7 (Rhabdomyosarcoma Marker) Mouse Monoclonal Antibody

catalog :

MBS438177

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[PAX7/497]

reactivity :

human, mouse, zebrafish

application :

western blot, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot of HeLa Cell Lysate using PAX7 Monoclonal Antibody (PAX7/497).

image 2 :

Purified PAX7 Mouse Monoclonal Antibody (PAX7/497).

product information

catalog number :

MBS438177

products type :

Antibody

products full name :

PAX7 (Rhabdomyosarcoma Marker) Mouse Monoclonal Antibody

products short name :

[PAX7]

products name syn :

[HUP1; Paired box gene 7; Paired box homeotic gene 7; Paired domain gene HuP1; PAX7 transcriptional factor; PAX7/FKHR fusion gene; PAX7B; RMS2]

other names :

[paired box protein Pax-7 isoform 3; Paired box protein Pax-7; paired box protein Pax-7; paired box 7; HuP1]

products gene name :

[PAX7]

other gene names :

[PAX7; PAX7; HUP1; RMS2; PAX7B; HUP1]

uniprot entry name :

PAX7_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[PAX7/497]

host :

Mouse

reactivity :

Human, Mouse, Rat Chicken, Zebrafish

sequence length :

505

specificity :

The Pax gene family of nuclear transcription factors is comprised of nine members that function during embryogenesis to regulate the temporal and position-dependent differentiation of cells. In addition, the family is involved in a variety of signal transduction pathways in the adult organism. Mutations in the Pax family of proteins have been linked to disease and cancer in humans. Pax-7 is a protein specifically expressed in cultured satellite cell-derived myoblasts. In situ hybridization reveals that Pax-7 is also expressed in satellite cells residing in adult muscle. A chromosomal aberration in the gene encoding Pax-7 causes rhabdomyosarcoma 2 (RMS2) (also called alveolar rhabdomyosarcoma).

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Immunofluorescence (IF), Flow Cytometry (FC/FACS), Western Blot (WB)

app notes :

Immunofluorescence (0.5-1ug/ml). Flow Cytometry (0.5-1ug/million cells in 0.1ml). Western Blot (0.5-1ug/ml). Optimal dilution for a specific application should be determined.

image1 heading :

Western Blot (WB)

image2 heading :

SDS-PAGE

other info1 :

Cellular Localization: Nuclear. Immunogen: Recombinant fragment (aa300-600) of human PAX7 protein

other info2 :

Hu-Chromosome Location: 1p36.13. Positive Control: HeLa, Jurkat, A431, MCF-7, U2OS or NIH/3T3 cells. Rhabdomyosarcoma (RMS)

ncbi gi num :

207029223

ncbi acc num :

NP_001128726.1

ncbi gb acc num :

NM_001135254.1

uniprot acc num :

P23759

ncbi mol weight :

57kDa

ncbi pathways :

Neural Crest Differentiation Pathway (672460); Transcriptional Misregulation In Cancer Pathway (523016); Transcriptional Misregulation In Cancer Pathway (522987); MiRs In Muscle Cell Differentiation Pathway (672449)

ncbi summary :

This gene is a member of the paired box (PAX) family of transcription factors. Members of this gene family typically contain a paired box domain, an octapeptide, and a paired-type homeodomain. These genes play critical roles during fetal development and cancer growth. The specific function of the paired box 7 gene is unknown but speculated to involve tumor suppression since fusion of this gene with a forkhead domain family member has been associated with alveolar rhabdomyosarcoma. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008]

uniprot summary :

PAX7: Probable transcription factor. May have a role in myogenesis. Defects in PAX7 are a cause of rhabdomyosarcoma type 2 (RMS2). It is a form of rhabdomyosarcoma, a highly malignant tumor of striated muscle derived from primitive mesenchimal cells and exhibiting differentiation along rhabdomyoblastic lines. Rhabdomyosarcoma is one of the most frequently occurring soft tissue sarcomas and the most common in children. It occurs in four forms: alveolar, pleomorphic, embryonal and botryoidal rhabdomyosarcomas. A chromosomal aberration involving PAX7 is found in rhabdomyosarcoma. Translocation t(1;13)(p36;q14) with FOXO1. The resulting protein is a transcriptional activator. Belongs to the paired homeobox family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: DNA-binding; Transcription factor; Oncoprotein. Chromosomal Location of Human Ortholog: 1p36.13. Molecular Function: transcription factor activity. Biological Process: anatomical structure morphogenesis; negative regulation of apoptosis. Disease: Rhabdomyosarcoma 2

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage