MART-1 / Melan-A / MLANA (Melanoma Marker) Mouse Monoclonal Antibody | MyBioSource MBS438176 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

MART-1 / Melan-A / MLANA (Melanoma Marker) Mouse Monoclonal Antibody

catalog :

MBS438176

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[A103 + M2-7C10 + M2-9E3]

reactivity :

human, mouse, rat, dog

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Melanoma stained with MART-1 / Melan-A Monoclonal Antibody (A103 + M2-7C10 + M2-9E3).

image 2 :

Formalin-fixed, paraffin-embedded human Testis stained with MART-1 / Melan-A Monoclonal Antibody (A103 + M2-7C10 + M2-9E3).

product information

catalog number :

MBS438176

products type :

Antibody

products full name :

MART-1 / Melan-A / MLANA (Melanoma Marker) Mouse Monoclonal Antibody

products short name :

[MART-1 / Melan-A / MLANA]

products name syn :

[Antigen LB39-AA, Antigen SK29-AA, Melanoma antigen recognized by T-cells 1, MLAN-A, MLANA]

other names :

[melanoma antigen recognized by T-cells 1; Melanoma antigen recognized by T-cells 1; melanoma antigen recognized by T-cells 1; melan-A; Antigen LB39-AA; Antigen SK29-AA; Protein Melan-A]

products gene name :

[MLANA]

other gene names :

[MLANA; MLANA; MART1; MART-1; MART1; MART-1]

uniprot entry name :

MAR1_HUMAN

clonality :

Monoclonal

isotype :

Mouse IgG1(A103)+ Mouse/IgG2b( M2-7C10 & M2-9E3).

clone :

[A103 + M2-7C10 + M2-9E3]

host :

Mouse

reactivity :

Human, Mouse, Rat, Dog. Others not tested

sequence length :

118

specificity :

This antibody recognizes a protein doublet of 20-22kDa, identified as MART-1 (Melanoma Antigen Recognized by T cells 1) or Melan-A. MART-1 is a newly identified melanocyte differentiation antigen recognized by autologous cytotoxic T lymphocytes. Seven other melanoma associated antigens recognized by autologous cytotoxic T cells include MAGE-1, MAGE-3, tyrosinase, gp100, gp75, BAGE-1, and GAGE-1. Subcellular fractionation shows that MART-1 is present in melanosomes and endoplasmic reticulum. This MAb cocktail labels melanomas and other tumors showing melanocytic differentiation. It is also a useful positive-marker for angiomyolipomas. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide store at 2-8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells in 0.1ml). Immunofluorescence (0.5-1ug/ml). Western Blot (0.5-1.0ug/ml). Immunohistology(Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

other info1 :

Cellular Localization: Cytoplasmic. Immunogen: Recombinant hMART-1 protein (A103; M2-7C10; M2-9E3)

other info2 :

Hu-Chromosome Location: 9p24.1. Positive Control: SK-MEL-13 and SK-MEL-19 Melanoma cell lines; Melanomas

products references :

Chen Y-T, et. al. Proc Natl Acad Sci, USA, 1996, 93:5915-19. Kawakami Y, et. al. Journal of Immunological Methods, 1997, 202(1):13-25. Marincola FM, et. al. Journal of Immunotherapy with Emphasis on Tumor Immunology, 1996, 19(3):192-205

ncbi gi num :

5031913

ncbi acc num :

NP_005502.1

ncbi gb acc num :

NM_005511.1

uniprot acc num :

Q16655

ncbi mol weight :

20-22kDa (doublet)

uniprot summary :

MLANA: Involved in melanosome biogenesis by ensuring the stability of GPR143. Plays a vital role in the expression, stability, trafficking, and processing of melanocyte protein PMEL, which is critical to the formation of stage II melanosomes. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 9p24.1. Cellular Component: endoplasmic reticulum membrane; Golgi apparatus; integral to plasma membrane; melanosome; trans-Golgi network. Molecular Function: protein binding

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage