CD11b | MyBioSource MBS438151 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD11b

catalog :

MBS438151

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

rat

conjugate :

nonconjugated

clone name :

[M1/70]

reactivity :

human, mouse, chimpanzee

application :

ELISA, immunohistochemistry, immunocytochemistry, immunoprecipitation, flow cytometry, FACS, enzyme immunoassay

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS438151

products type :

Antibody

products full name :

CD11b

products short name :

[CD11b]

products name syn :

[CD11 antigen-like family member B; CD11b/CD18; CD49d; Cell surface glycoprotein MAC-1 subunit alpha; Complement Component Receptor 3 Alpha; CR3 Alpha Chain (CR3A); Integrin alpha-M; Integrin beta 2 alpha subunit; ITGAM; Leukocyte adhesion receptor MO1; Ly-40; Mac-1a; MAC1; Mac1; alpha subunit; MAC1A; Macrophage antigen alpha polypeptide; MO1A; Neutrophil adherence receptor alpha M subunit]

other names :

[integrin alpha-M isoform 2; Integrin alpha-M; integrin alpha-M; CR-3 alpha chain; antigen CD11b (p170); leukocyte adhesion receptor MO1; CD11 antigen-like family member B; macrophage antigen alpha polypeptide; cell surface glycoprotein MAC-1 subunit alpha; neutrophil adherence receptor alpha-M subunit; integrin, alpha M (complement component 3 receptor 3 subunit); CD11 antigen-like family member B; CR-3 alpha chain; Cell surface glycoprotein MAC-1 subunit alpha; Leukocyte adhesion receptor MO1; Neutrophil adherence receptor; CD_antigen: CD11b]

products gene name :

[ITGAM]

other gene names :

[ITGAM; ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6; CD11B; CR3A]

uniprot entry name :

ITAM_HUMAN

clonality :

Monoclonal

isotype :

IgG2b, kappa

clone :

[M1/70]

host :

Rat

reactivity :

Human, Chimpanzee, Baboon, Cynomolgus, Rhesus, Rabbit and Mouse.

sequence length :

1152

specificity :

CD11b is a cell adhesion molecule that acts as a receptor for cell surface ligands such as intracellular adhesion molecules (ICAMs) or soluble ligands. Integrins are heterodimeric proteins that contain an a chain and b chain. Integrin åM combines with the Integrin ?2 to form a leukocyte-specific integrin referred to as macrophage receptor 1 (Mac-1), or inactivated-C3b (iC3b) receptor 3 (CR3). Integrin åM/?2 is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles.The protein CD11b has been implicated in the various adhesion-related interactions of cells such as monocytes, macrophages, natural killer (NK) cells, and granulocytes. It is part of a heterodimer that consists of CD11b and CD18. It also modulates the uptake of complement-coated particles within the cell. It is commonly used as a microglial marker in tissues derived from the nervous system.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Store at 2 to 8 degree C. Antibody is stable for 24 months.

tested application :

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Functional Studies , Immunoprecipitation (IP), Immunohistology (IH)

app notes :

ELISA (For coating, order Ab without BSA) Flow Cytometry (0.5-1ug/million cells); Immunofluorescence (0.5-1ug/ml); Functional Studies (Order Ab without BSA Immunoprecipitation (0.5-1ug/500ug protein lysate); Immunohistology (Frozen) (0.5-1.0ug/ml for 30 minutes at RT) Optimal dilution for a specific application should be determined.

other info1 :

Localization: Cell surface. Positive Control: Monocytes & granulocytes. Lymph nodes and tonsils. Human Chromosomal Location: 16p11.2

other info2 :

Immunogen: B10 mouse spleen cells enriched for T lymphocytes. Unigene: 172631 (Human)

products references :

1. Springer T, et al. 1978. Eur. J. Immunol. 8:539. 2. Ault K and Springer T. 1981. J. Immunol. 126:359. 3. Springer TA, et al. 1982. Immunol. Rev. 68:171. 4. Ho MK and Springer TA. 1983. J. Biol. Chem. 258:2766. 5. Flotte TJ, et al. 1983. Am. J. Pathol. 111:112.

ncbi gi num :

88501734

ncbi acc num :

NP_000623.2

ncbi gb acc num :

NM_000632.3

uniprot acc num :

P11215

ncbi mol weight :

95kDa; 170kDa

ncbi pathways :

Amoebiasis Pathway (167324); Amoebiasis Pathway (167191); Cell Adhesion Molecules (CAMs) Pathway (83069); Cell Adhesion Molecules (CAMs) Pathway (480); Cell Surface Interactions At The Vascular Wall Pathway (106062); Extracellular Matrix Organization Pathway (576262); Focal Adhesion Pathway (198795); Hematopoietic Cell Lineage Pathway (83078); Hematopoietic Cell Lineage Pathway (489); Hemostasis Pathway (106028)

ncbi summary :

This gene encodes the integrin alpha M chain. Integrins are heterodimeric integral membrane proteins composed of an alpha chain and a beta chain. This I-domain containing alpha integrin combines with the beta 2 chain (ITGB2) to form a leukocyte-specific integrin referred to as macrophage receptor 1 ('Mac-1'), or inactivated-C3b (iC3b) receptor 3 ('CR3'). The alpha M beta 2 integrin is important in the adherence of neutrophils and monocytes to stimulated endothelium, and also in the phagocytosis of complement coated particles. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2009]

uniprot summary :

ITGAM: a single-pass type I membrane protein of the integrin alpha chain family. Implicated in various adhesive interactions of monocytes, macrophages and granulocytes as well as in mediating the uptake of complement-coated particles. Also known as CR-3, the receptor for the iC3b fragment of the third complement component. It probably recognizes the RGD peptide in C3b. A receptor for fibrinogen, factor X and ICAM1. Heterodimer of an alpha and a beta subunit. Alpha-M associates with beta-2. Predominantly expressed in monocytes and granulocytes. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, integral. Chromosomal Location of Human Ortholog: 16p11.2. Cellular Component: cell surface; plasma membrane; nucleus; integrin complex; external side of plasma membrane. Molecular Function: heparan sulfate proteoglycan binding; heparin binding; opsonin binding; protein binding; protein heterodimerization activity; metal ion binding; glycoprotein binding. Biological Process: integrin-mediated signaling pathway; neutrophil chemotaxis; extracellular matrix organization and biogenesis; microglia development; cellular extravasation; activated T cell proliferation; toll-like receptor signaling pathway; innate immune response; cell adhesion; blood coagulation; toll-like receptor 4 signaling pathway; leukocyte migration; leukocyte migration during inflammatory response. Disease: Systemic Lupus Erythematosus, Susceptibility To, 6

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage