CD68 (Macrophage Marker) Mouse Monoclonal Antibody | MyBioSource MBS438131 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD68 (Macrophage Marker) Mouse Monoclonal Antibody

catalog :

MBS438131

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

reactivity :

human, mouse, rat

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Histiocytoma stained with CD68 Monoclonal Antibody (C68/684).

image 2 :

Formalin-fixed, paraffin-embedded human Tonsil stained with CD68 Monoclonal Antibody (C68/684).

image 3 :

SDS-PAGE Analysis of Purified CD68 Monoclonal Antibody (C68/684).

product information

catalog number :

MBS438131

products type :

Antibody

products full name :

CD68 (Macrophage Marker) Mouse Monoclonal Antibody

products short name :

[CD68]

products name syn :

[GP110, LAMP4, Microsialin, Macrosialin, SCARD1, Scavenger Receptor Class D Member-1]

other names :

[macrosialin isoform B; Macrosialin; macrosialin; CD68 molecule; Gp110; CD_antigen: CD68]

products gene name :

[CD68]

other gene names :

[CD68; CD68; GP110; LAMP4; SCARD1]

uniprot entry name :

CD68_HUMAN

clonality :

isotype :

IgG1, kappa

host :

Mouse

reactivity :

Human, Monkey, Mouse, and Rat. Others not known.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Store at 2-8°C.

tested application :

Western Blot (WB), Immunofluorescence (IF), Flow Cytometry (FC), Immunohistology (Formalin-fixed)

app notes :

Western blotting (1-2ug/ml). Immunofluorescence (1-2ug/ml). Flow Cytometry (1-2ug/million cells). Immunohistology (Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

SDS-PAGE

image4 heading :

Western Blot (WB)

image4 description :

Western Blot Analysis of human Spleen tissue lysate using CD68 Monoclonal Antibody (C68/684).

image5 heading :

Flow Cytometry (FC)

image5 description :

Flow Cytometric Analysis of U87MG cells using CD68 Monoclonal Antibody (C68/684); followed by goat anti-mouse IgG-CF488 (Blue); Isotype Control (Red).

other info1 :

Human Unigene: 647419. Hu-Chromosome Location: 17p13.1. Immunogen: Recombinant human CD68 protein. Cellular Localization: Cell Surface and Cytoplasmic. Positive Control: U87MG cells,Tonsil, Lymph Node or Spleen

products description :

This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.

products references :

Pulford KA et. al. Journal of Clinical Pathology, 1989, 42(4):414-21. Warnke RA et. al. Am J of Pathol, 1989, 135:1089-95.

ncbi gi num :

91199550

ncbi acc num :

NP_001035148.1

ncbi gb acc num :

NM_001040059.1

uniprot acc num :

P34810

ncbi mol weight :

110kDa

ncbi pathways :

Lysosome Pathway (99052); Lysosome Pathway (96865)

ncbi summary :

This gene encodes a 110-kD transmembrane glycoprotein that is highly expressed by human monocytes and tissue macrophages. It is a member of the lysosomal/endosomal-associated membrane glycoprotein (LAMP) family. The protein primarily localizes to lysosomes and endosomes with a smaller fraction circulating to the cell surface. It is a type I integral membrane protein with a heavily glycosylated extracellular domain and binds to tissue- and organ-specific lectins or selectins. The protein is also a member of the scavenger receptor family. Scavenger receptors typically function to clear cellular debris, promote phagocytosis, and mediate the recruitment and activation of macrophages. Alternative splicing results in multiple transcripts encoding different isoforms. [provided by RefSeq, Jul 2008]

uniprot summary :

CD68: Could play a role in phagocytic activities of tissue macrophages, both in intracellular lysosomal metabolism and extracellular cell-cell and cell-pathogen interactions. Binds to tissue- and organ-specific lectins or selectins, allowing homing of macrophage subsets to particular sites. Rapid recirculation of CD68 from endosomes and lysosomes to the plasma membrane may allow macrophages to crawl over selectin-bearing substrates or other cells. Belongs to the LAMP family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Cell surface. Chromosomal Location of Human Ortholog: 17p13. Cellular Component: membrane

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage