CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody | MyBioSource MBS438089 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody

catalog :

MBS438089

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[SPM524]

reactivity :

human, mouse

application :

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western Blot of human Spleen Lysate with CD63 Monoclonal Antibody (SPM524)

image 2 :

Formalin-fixed, paraffin-embedded human Melanoma stained with CD63 Monoclonal Antibody (SPM524) (AEC Chromogen)

image 3 :

Formalin-fixed, paraffin-embedded Mouse Spleen stained with CD63 Monoclonal Antibody (SPM524) (DAB Chromogen)

product information

catalog number :

MBS438089

products type :

Antibody

products full name :

CD63 (Late Endosomes Marker) Mouse Monoclonal Antibody

products short name :

[CD63]

products name syn :

[gp55; granulophysin; Lysosomal-associated membrane protein 3 (LAMP-3); Mast cell antigen AD1; melanoma 1 antigen; Melanoma-associated antigen MLA1; Melanoma-associated antigen ME491; MLA1; NGA; Ocular melanoma-associated antigen; OMA81H; PTLGP40; Tetraspanin-30; TSPAN30]

other names :

[CD63 antigen isoform A; CD63 antigen; CD63 antigen; CD63 molecule; Granulophysin; Lysosomal-associated membrane protein 3; LAMP-3; Melanoma-associated antigen ME491; OMA81H; Ocular melanoma-associated antigen; Tetraspanin-30; Tspan-30; CD_antigen: CD63]

products gene name :

[CD63]

other gene names :

[CD63; CD63; MLA1; ME491; LAMP-3; OMA81H; TSPAN30; MLA1; TSPAN30; LAMP-3; Tspan-30]

uniprot entry name :

CD63_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[SPM524]

host :

Mouse

reactivity :

Human, Mouse

sequence length :

238

specificity :

The tetraspanins are integral membrane proteins expressed on cell surface and granular membranes of hematopoietic cells and are components of multi-molecular complexes with specific integrins. The tetraspanin CD63 is a lysosomal membrane glycoprotein that translocates to the plasma membrane after platelet activation. CD63 is expressed on activated platelets, monocytes and macrophages, and is weakly expressed on granulocytes, T cell and B cells. It is located on the basophilic granule membranes and on the plasma membranes of lymphocytes and granulocytes. CD63 is a member of the TM4 superfamily of leukocyte glycoproteins that includes CD9, CD37 and CD53, which contain four transmembrane regions. CD63 may play a role in phagocytic and intracellular lysosome-phagosome fusion events. CD63 deficiency is associated with Hermansky-Pudlak syndrome and is strongly expressed during the early stages of melanoma progression.

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide store at 2-8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

app notes :

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.5-1ug/ml). Western Blotting (0.5-1ug/ml). Immunohistology(Formalin-fixed) (0.5-1ug/ml for 30 minutes at RT) . (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.

image1 heading :

Western Blot (WB)

image2 heading :

Immunohistochemistry (IHC)

image3 heading :

Immunohistochemistry (IHC)

image4 heading :

Flow Cytometry (FC)

image4 description :

Flow Cytometry of human CD63 on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD63 Monoclonal Antibody (NKI/C3).

other info1 :

Cellular Localization: Cell Surface & Cytoplasmic. Immunogen: Smooth plasma membrane fraction of MeWo cells

other info2 :

Hu-Chromosome Location: 12q13.2. Positive Control: SK-MEL-28, HL60, THP-1 or NIH/3T3 cells. Melanoma or lymphoma.

products references :

C. Vennegoor et al., Int. J. Cancer 35: 287-295, 1985. AA Palmer et al., Pathology 17: 335-339, 1985. EC Hagen et al., Histopathology 10: 689-700, 1986

ncbi gi num :

383872447

ncbi acc num :

NP_001244318.1

ncbi gb acc num :

NM_001257389.1

uniprot acc num :

P08962

ncbi mol weight :

26kDa (core protein); 30-60kDa (glycosylated)

ncbi pathways :

Hemostasis Pathway (1269340); Lysosome Pathway (99052); Lysosome Pathway (96865); Platelet Activation, Signaling And Aggregation Pathway (1269350); Platelet Degranulation Pathway (1269367); Proteoglycans In Cancer Pathway (782000); Proteoglycans In Cancer Pathway (782054); Response To Elevated Platelet Cytosolic Ca2+ Pathway (1269365)

ncbi summary :

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. The encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. Alternative splicing results in multiple transcript variants encoding different protein isoforms. [provided by RefSeq, Apr 2012]

uniprot summary :

CD63: This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation. Belongs to the tetraspanin (TM4SF) family. Protein type: Membrane protein, integral; Membrane protein, multi-pass. Chromosomal Location of Human Ortholog: 12q12-q13. Cellular Component: cell surface; endosome membrane; extracellular space; integral to plasma membrane; intrinsic to plasma membrane; late endosome membrane; lysosomal membrane; plasma membrane; platelet dense granule membrane. Molecular Function: protein binding. Biological Process: cell migration; cell-matrix adhesion; pigment granule maturation; platelet degranulation; positive regulation of receptor internalization

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage