antibody

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody

MBS438088

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

polyclonal

rabbit

nonconjugated

human, mouse, rat, cow

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

Antibody

p40 (deltaNp63) (Squamous, Basal & Myoepithelial Cell Marker) Rabbit Polyclonal Antibody

[p40 (deltaNp63)]

[deltaNp63, TP63]

[tumor protein 63 isoform 2; Tumor protein 63; tumor protein 63; tumor protein p63; Chronic ulcerative stomatitis protein; CUSP; Keratinocyte transcription factor KET; Transformation-related protein 63; TP63; Tumor protein p73-like; p73L; p40; p51]

[TP63]

[TP63; TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B); KET; P63; P73H; P73L; TP73L; p63; CUSP; TP63; p73L]

P63_HUMAN

Polyclonal

IgG

Rabbit

Human, Mouse, Rat, Cow. Others not known.

555

p40 (p63 delta) is a marker recently determined to be highly specific for squamous basal cells in the immunohistochemistry (IHC) application. The current more routinely recommended marker, p63, appears to have less specificity compared to p40, especially on squamous cell tumors. The ability to differentiate between lung adenocarcinoma vs. squamous cell carcinoma is difficult and has bearing on the different therapeutic avenues for each subtype treatment. p63 antibody's ability to distinguish between the tumor types appears to be inferior when compared to p40. The ability to utilize an antibody probe for p40 as a squamous cell marker bolsters its use for future sub-classification of lung cancers, especially by immunohistochemical techniques.

200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20° to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) Formalin

Flow Cytometry (1-2ug/million cells in 0.1ml). Immunofluorescence (1-2ug/ml). Western Blot (1-2ug/ml). Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilutions for a specific application should be determined.

Immunohistochemistry (IHC)

Immunohistochemistry

Western Blot

SDS-PAGE

Cellular Localization: Nuclear. Immunogen: A synthetic peptide (ENNAQTQFSEPQY) corresponding to aa5-17 of human p40

Hu-Chromosome Location: 3q28. Positive Control: HEK293 cells or Prostate Carcinoma or Lung Squamous Cell Carcinoma

Bishop, JA et. al. Modern Pathology 25 : 405-4152. Scagliotti G et. al. J Thorac Oncol 6:64-70. 3. Kargi A et. al. Appl Immunohistochem Mol Morphol 15:415-420

169234657

NP_001108450.1

NM_001114978.1

Q9H3D4

40kDa

Activation Of BH3-only Proteins Pathway (1270270); Activation Of PUMA And Translocation To Mitochondria Pathway (1270273); Apoptosis Pathway (198797); Apoptosis Pathway (1270262); Direct P53 Effectors Pathway (137939); Gene Expression Pathway (1269649); Generic Transcription Pathway (1269650); Intrinsic Pathway For Apoptosis (1270268); MicroRNAs In Cancer Pathway (852705); MicroRNAs In Cancer Pathway (852928)

This gene encodes a member of the p53 family of transcription factors. An animal model, p63 -/- mice, has been useful in defining the role this protein plays in the development and maintenance of stratified epithelial tissues. p63 -/- mice have several developmental defects which include the lack of limbs and other tissues, such as teeth and mammary glands, which develop as a result of interactions between mesenchyme and epithelium. Mutations in this gene are associated with ectodermal dysplasia, and cleft lip/palate syndrome 3 (EEC3); split-hand/foot malformation 4 (SHFM4); ankyloblepharon-ectodermal defects-cleft lip/palate; ADULT syndrome (acro-dermato-ungual-lacrimal-tooth); limb-mammary syndrome; Rap-Hodgkin syndrome (RHS); and orofacial cleft 8. Both alternative splicing and the use of alternative promoters results in multiple transcript variants encoding different proteins. [provided by RefSeq, Jul 2016]

p63: Acts as a sequence specific DNA binding transcriptional activator or repressor. The isoforms contain a varying set of transactivation and auto-regulating transactivation inhibiting domains thus showing an isoform specific activity. Isoform 2 activates RIPK4 transcription. May be required in conjunction with TP73/p73 for initiation of p53/TP53 dependent apoptosis in response to genotoxic insults and the presence of activated oncogenes. Involved in Notch signaling by probably inducing JAG1 and JAG2. Plays a role in the regulation of epithelial morphogenesis. The ratio of DeltaN-type and TA*-type isoforms may govern the maintenance of epithelial stem cell compartments and regulate the initiation of epithelial stratification from the undifferentiated embryonal ectoderm. Required for limb formation from the apical ectodermal ridge. Activates transcription of the p21 promoter. Binds DNA as a homotetramer. Isoform composition of the tetramer may determine transactivation activity. Isoforms Alpha and Gamma interact with HIPK2. Interacts with SSRP1, leading to stimulate coactivator activity. Isoform 1 and isoform 2 interact with WWP1. Interacts with PDS5A. Isoform 5 (via activation domain) interacts with NOC2L. Widely expressed, notably in heart, kidney, placenta, prostate, skeletal muscle, testis and thymus, although the precise isoform varies according to tissue type. Progenitor cell layers of skin, breast, eye and prostate express high levels of DeltaN-type isoforms. Isoform 10 is predominantly expressed in skin squamous cell carcinomas, but not in normal skin tissues. Belongs to the p53 family. 12 isoforms of the human protein are produced by alternative promoter. Protein type: Transcription factor; DNA-binding. Chromosomal Location of Human Ortholog: 3q28. Cellular Component: cytoplasm; cytosol; dendrite; nuclear chromatin; nucleoplasm; nucleus; transcription factor complex. Molecular Function: damaged DNA binding; double-stranded DNA binding; identical protein binding; p53 binding; protein binding; sequence-specific DNA binding; transcription factor activity; WW domain binding. Biological Process: apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis; DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator; G1 DNA damage checkpoint; negative regulation of apoptosis; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of Notch signaling pathway; positive regulation of osteoblast differentiation; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; protein homotetramerization; regulation of apoptosis; regulation of epidermal cell division; regulation of neuron apoptosis; response to gamma radiation; response to X-ray. Disease: Adult Syndrome; Ankyloblepharon-ectodermal Defects-cleft Lip/palate; Ectrodactyly, Ectodermal Dysplasia, And Cleft Lip/palate Syndrome 3; Limb-mammary Syndrome; Rapp-hodgkin Syndrome; Split-hand/foot Malformation 4

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA at 1mg/ml)

340