catalog number :
MBS4380765
products full name :
Lambda Light Chain (B-Cell Marker) Recombinant Rabbit Monoclonal Antibody [Clone LLC/3778R]
products short name :
[Lambda Light Chain (B-Cell Marker)]
products name syn :
[Bence Jones Protein; BJP; IGLC 1/2/3; Mcg Marker; Paraprotein]
reactivity :
Human. Others not known.
specificity :
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
form :
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
storage stability :
Antibody with azide: store at 2-8°C. Antibody without azide: store at -20°C to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
tested application :
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC) (Formalin-fixed)
app notes :
Flow Cytometry (1-2ug/million cells); Immunofluorescence (1-2ug/ml); Western Blot (1-2ug/ml); Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT) (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes) Optimal dilution for a specific application should be determined.
image1 heading :
Immunohistochemistry (IHC)
image2 heading :
Immunohistochemistry (IHC)
image3 heading :
SDS-Page
other info1 :
Human Unigene: 449585. Human Gene Symbol: IGL. Human Chromosome Location: 22q11.22
other info2 :
Immunogen: Recombinant full-length human IGL protein. Cellular Localization: Cell Surface, cytoplasmic and secreted. Positive Control: 293T, Raji or hPBL cells. Tonsil or Spleen.
ncbi mol weight :
~22.5kDa
size1 :
0.02 mg (With BSA & Azide at 0.2 mg/ml)
size2 :
0.1 mg (With BSA & Azide at 0.2mg/ml)
size3 :
0.1 mg (Without BSA & Azide at 1mg/ml)