catalog number :
MBS4380759
products full name :
Mitochondria (Marker for Human Cells, Granular RCCs & Salivary Tumors)
products short name :
[Mitochondria]
reactivity :
Human. Does not react with mouse, rat. Others not known
specificity :
This MAb recognizes a 60kDa antigen associated with the mitochondria in human cells. It can be used to stain mitochondria in cell or tissue preparations and can be used as a mitochondrial marker in subcellular fractions. It produces a spaghetti-like pattern in normal and malignant cells. This antibody is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells, including neurons and embryonic stem cells. Immunostaining pattern with anti-mitochondrial MAb has been reported as a useful discriminatory adjunct in the complex differential diagnosis of granular renal cell tumors. Reportedly, this MAb facilitates the classification of salivary tumors.
purity :
Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
form :
200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
storage stability :
Antibody with azide - store at 2 to 8°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
tested application :
Immunohistology (Formalin-Fixed)
app notes :
Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 minutes at RT. (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris buffer with 1mM EDTA, pH9.0, for 10-20 min followed by cooling at RT for 20 minutes). Optimal dilution for a specific application should be determined.
image1 heading :
Immunohistochemistry
image2 heading :
SDS-PAGE
other info1 :
Immunogen: Semi-purified mitochondrial preparation. Positive Control: HeLa or HepG2 Cells. Hepatic carcinoma.
other info2 :
Cellular Localization: Mitochondria in cytoplasm
products description :
Recombinant Rabbit Monoclonal Antibody
products references :
Tickoo SK, et al. Am J Surg Pathol. 1997; 21(8):922-30.; Vera-Sempere F, et al. Acta Otorrinolaringol Esp. 2011; 62(1):1-9.
size1 :
0.02 mg (With BSA & Azide at 0.2 mg/ml)
size2 :
0.1 mg (With BSA & Azide at 0.2mg/ml)
size3 :
0.1 mg (Without BSA & Azide at 1mg/ml)