Perforin-1 (Pore Forming Protein) (Apoptosis Marker) | MyBioSource MBS4380665 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

Perforin-1 (Pore Forming Protein) (Apoptosis Marker)

catalog :

MBS4380665

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[PRF1/2470]

reactivity :

human

application :

immunohistochemistry

more info or order :

MyBioSource product webpage

image

image 1 :

Formalin-fixed, paraffin-embedded human Spleen stained with Perforin-1 Mouse Monoclonal Antibody (PRF1/2470).

image 2 :

Analysis of Protein Array containing more than 19,000 full-length human proteins using Perforin-1 Mouse Monoclonal Antibody (PRF1/2470).Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

image 3 :

SDS-PAGE Analysis Purified Perforin-1 Mouse Monoclonal Antibody (PRF1/2470).(Confirmation of Integrity and Purity of Antibody

product information

catalog number :

MBS4380665

products type :

Antibody

products full name :

Perforin-1 (Pore Forming Protein) (Apoptosis Marker)

products short name :

[Perforin-1]

products name syn :

[Cytolysin; FLH2; HPLH2; Lymphocyte pore-forming protein; PRF1 (pore forming protein 1); Perforin-1; PFP; PGFL; PIGF; PIGF-2; PLGF]

other names :

[perforin-1; Perforin-1; perforin-1; perforin 1; Cytolysin; Lymphocyte pore-forming protein; PFP]

products gene name :

[PRF1]

other gene names :

[PRF1; PRF1; P1; PFP; HPLH2; PFP; P1; PFP]

clonality :

Monoclonal

isotype :

IgG2c, kappa

clone :

[PRF1/2470]

host :

Mouse

reactivity :

Human. Others not known.

sequence length :

555

specificity :

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis. Perforin has structural and functional similarities to complement component 9 (C9). Like C9, this protein creates transmembrane tubules and is capable of lysing non-specifically a variety of target cells. It is one of the main cytolytic proteins of cytolytic granules, and is known to be a key effector molecule for T-cell- and natural killer-cell-mediated cytolysis. Defects in this gene cause familial hemophagocytic lymphohistiocytosis type 2 (HPLH2), a rare and lethal autosomal recessive disorder of early childhood. The expression of perforin is reportedly upregulated in activated CD8+ T-cells, natural killer cells and some CD4+ T-cells.

purity :

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Immunohistology (Formalin-Fixed)

app notes :

Immunohistology (Formalin-Fixed): 1-2ug/ml for 30 min at RT; Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes. Optimal dilution for a specific application should be determined.

image1 heading :

Immunohistochemistry (IHC)

image2 heading :

Testing Data

image3 heading :

SDS-PAGE

other info1 :

Immunogen: Recombinant human Perforin-1 protein fragment (around aa 413-552) (exact sequence is proprietary). Positive Control: HDLM-2 Cells. Spleen

other info2 :

Cellular Localization: Cytoplasmic. Chromosome Location: 10q22.1

products references :

Bittmann I, et al. Fas/FasL and perforin/granzyme pathway in acute rejection and diffuse alveolar damage after allogeneic lung transplantation- a human biopsy study. Virchows Arch. 2004; 445:375-81.

ncbi gi num :

133908621

ncbi acc num :

NP_001076585.1

ncbi gb acc num :

NM_001083116.2

uniprot acc num :

P14222

ncbi mol weight :

75kDa

ncbi summary :

This gene encodes a protein with structural similarities to complement component C9 that is important in immunity. This protein forms membrane pores that allow the release of granzymes and subsequent cytolysis of target cells. Whether pore formation occurs in the plasma membrane of target cells or in an endosomal membrane inside target cells is subject to debate. Mutations in this gene are associated with a variety of human disease including diabetes, multiple sclerosis, lymphomas, autoimmune lymphoproliferative syndrome (ALPS), aplastic anemia, and familial hemophagocytic lymphohistiocytosis type 2 (FHL2), a rare and lethal autosomal recessive disorder of early childhood. [provided by RefSeq, Aug 2017]

uniprot summary :

Plays a key role in secretory granule-dependent cell death, and in defense against virus-infected or neoplastic cells. Plays an important role in killing other cells that are recognized as non-self by the immune system, e.g. in transplant rejection or some forms of autoimmune disease. Can insert into the membrane of target cells in its calcium-bound form, oligomerize and form large pores. Promotes cytolysis and apoptosis of target cells by facilitating the uptake of cytotoxic granzymes.

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA & Azide at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage