CD3e (T-Cell Marker) | MyBioSource MBS438059 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD3e (T-Cell Marker)

catalog :

MBS438059

quantity :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price :

190 USD

clonality :

polyclonal

host :

rabbit

conjugate :

nonconjugated

reactivity :

human, mouse, rat, dog, chicken, cow, chimpanzee, Rhesus monkey

application :

western blot, immunocytochemistry, immunoprecipitation, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

Western blot of CD3e in human Jurkat cells . 1) absence . 2) presence of immunizing peptide. 3) Mouse thymus probed with CD3e Rabbit PAb.

image 2 :

Western Blot of lurkat Cell Lysate using CD3e Rabbit Polyclonal Antibody.

product information

catalog number :

MBS438059

products type :

Antibody

products full name :

CD3e (T-Cell Marker)

products short name :

[CD3e]

products name syn :

[CD 3E; CD3 epsilon; CD3 TCR complex; CD3E; CD3e antigen epsilon polypeptide (TiT3 complex); T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex]

other names :

[T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; CD3-epsilon; T-cell surface antigen T3/Leu-4 epsilon chain; CD3e antigen, epsilon polypeptide (TiT3 complex); T-cell antigen receptor complex, epsilon subunit of T3; CD3e molecule, epsilon (CD3-TCR complex); T-cell surface antigen T3/Leu-4 epsilon chain; CD_antigen: CD3e]

products gene name :

[CD3E]

other gene names :

[CD3E; CD3E; T3E; TCRE; IMD18; T3E]

uniprot entry name :

CD3E_HUMAN

clonality :

Polyclonal

isotype :

Immunoglobulin

host :

Rabbit

reactivity :

Human. Predict reacted with: Elephant, gibbon, Guinea Pig, Hamster, Horse, Macaque, Mole Rat, Monkey, Mouse, New World monkey, Orangutan, Pig, Rabbit, Rat, Rhesus Monkey, Sheep, Ape, Cat, Chicken, Chimpanzee, Cow, Dog, Frog, Opossum.

sequence length :

207

specificity :

Recognizes the chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

form :

Supplied as 200ug/ml of Ab purified from rabbit anti-serum by Protein A. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available Without BSA at 1.0mg/ml.

storage stability :

Antibody with azide-Store at 2 to 8 degree C. Antibody without azide-store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

tested application :

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunoprecipitation (IP)

app notes :

Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (1-2ug/ml). Western Blotting (1-2ug/ml for 60 minutes at RT). Immunoprecipitation (1-2ug/500ug protein lysate). Optimal dilution for a specific application should be determined.

image1 heading :

Testing Data

image2 heading :

Western Blot

other info1 :

Localization: Cell surface and cytoplasmic. Positive Control: Jurkat cells. Tonsil and lymph node. Human Chromosomal Location: 11q23.3

other info2 :

Immunogen: A synthetic peptide aa156-168 (ERPPPVPNPDYEP) from human CD3e. Quantity and Buffer: 0.5 ml at 200ug/ml with BSA and azide

products references :

1. Cibull ML et. al. Histopathology, 1989, 15(6):599-605. 2. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

ncbi gi num :

4502671

ncbi acc num :

NP_000724.1

ncbi gb acc num :

NM_000733.3

uniprot acc num :

P07766

ncbi mol weight :

20kDa

ncbi pathways :

Adaptive Immune System Pathway (366160); CXCR4-mediated Signaling Events Pathway (137910); Chagas Disease (American Trypanosomiasis) Pathway (147809); Chagas Disease (American Trypanosomiasis) Pathway (147795); Costimulation By The CD28 Family Pathway (119552); Downstream TCR Signaling Pathway (106418); Downstream Signaling In Naive CD8+ T Cells Pathway (138018); Generation Of Second Messenger Molecules Pathway (106417); HTLV-I Infection Pathway (373901); HTLV-I Infection Pathway (373889)

ncbi summary :

The protein encoded by this gene is the CD3-epsilon polypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma and delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. [provided by RefSeq, Jul 2008]

uniprot summary :

CD3E: a T cell surface glycoprotein that is a component of the T cell antigen receptor. The recruitment of Nck by CD3 epsilon reveals a ligand-induced conformational change essential for T cell receptor signaling and synapse formation. Contains 1 immunoglobulin-like domain and 1 ITAM domain. Protein type: Receptor, misc.; Membrane protein, integral. Chromosomal Location of Human Ortholog: 11q23. Cellular Component: T cell receptor complex; integral to plasma membrane; plasma membrane; immunological synapse; alpha-beta T cell receptor complex; intercellular junction; external side of plasma membrane. Molecular Function: protein binding; receptor signaling complex scaffold activity; transmembrane receptor activity; protein heterodimerization activity; receptor signaling protein activity; T cell receptor binding; protein kinase binding; SH3 domain binding. Biological Process: regulation of immune response; T cell activation; positive regulation of interleukin-2 biosynthetic process; signal complex assembly; positive regulation of calcium-mediated signaling; negative thymic T cell selection; T cell receptor signaling pathway; regulation of apoptosis; positive regulation of interleukin-4 production; G-protein coupled receptor protein signaling pathway; positive regulation of interferon-gamma production; positive regulation of peptidyl-tyrosine phosphorylation; cell surface receptor linked signal transduction; negative regulation of smoothened signaling pathway; T cell costimulation; positive regulation of T cell proliferation; protein complex assembly; positive regulation of alpha-beta T cell proliferation; positive regulation of T cell anergy; transmembrane receptor protein tyrosine kinase signaling pathway; response to nutrient. Disease: Immunodeficiency 18

size1 :

0.02 mg (With BSA & Azide at 0.2 mg/ml)

price1 :

190 USD

size2 :

0.1 mg (With BSA & Azide at 0.2mg/ml)

price2 :

340

size3 :

0.1 mg (Without BSA at 1mg/ml)

price3 :

340

more info or order :

MyBioSource product webpage