antibody

ACTH

MBS438058

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[57]

human, mouse, rat

ELISA, immunohistochemistry, immunocytochemistry, flow cytometry, FACS, enzyme immunoassay

Antibody

ACTH

[ACTH]

[Adrenocorticotropin; alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin; beta-Endorphin; beta or gamma Lipotropin (LPH); CLIP; Met Enkephalin; POC; POMC]

[pro-opiomelanocortin preproprotein; Pro-opiomelanocortin; pro-opiomelanocortin; beta-LPH; beta-MSH; alpha-MSH; gamma-LPH; gamma-MSH; beta-endorphin; met-enkephalin; lipotropin beta; lipotropin gamma; melanotropin beta; melanotropin alpha; melanotropin gamma; pro-ACTH-endorphin; adrenocorticotropin; corticotropin-lipotropin; adrenocorticotropic hormone; opiomelanocortin prepropeptide; proopiomelanocortin preproprotein; beta-melanocyte-stimulating hormone; alpha-melanocyte-stimulating hormone; corticotropin-like intermediary peptide; proopiomelanocortin; Corticotropin-lipotropinCleaved into the following 11 chains:NPP; Melanotropin gammaAlternative name(s):Gamma-MSH]

[POMC]

[POMC; POMC; LPH; MSH; NPP; POC; ACTH; CLIP; POMC; ACTH; CLIP]

COLI_HUMAN

Monoclonal

IgG1, kappa

[57]

Mouse

Human, Mouse, Rat. Expected to show a broad species reactivity.

267

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

Store at 2 to 8 degree C. Antibody is stable for 24 months.

ELISA (EIA), Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistology (IH)

ELISA (For coating, order Ab without BSA). Flow Cytometry (0.5-1ug/million cells). Immunofluorescence (0.5-1ug/ml). Immunohistology (Frozen & Formalin-fixed) (0.5-1.0ug/ml for 30 minutes at RT) (No special pretreatment is required for staining of formalin-fixed tissues). Optimal dilution for a specific application should be determined.

SDS-PAGE

Immunohistochemistry

Positive Control: Normal pituitary gland or pituitary tumor. Human Chromosomal Location: 2p23.3

Immunogen: N-terminal fragment of human ACTH conjugated to KLH. Cellular Localization: Cytoplasm and Secreted

1. Hsu DW et. al. American Journal of Pathology, 1991, 138(4):897-909.

4505949

NP_000930.1

NM_000939.2

P01189

ACTH is ~5kDa; the POMC precursor is ~30kDa. Note: The molecular weight of POMC depends upon isoform variation and post-translational modifications.

Adipocytokine Signaling Pathway (83093); Adipocytokine Signaling Pathway (505); Androgen Biosynthesis Pathway (106154); Biological Oxidations Pathway (105698); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Corticotropin-releasing Hormone Pathway (920957); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Endogenous Sterols Pathway (105701); G Alpha (i) Signalling Events Pathway (119550); G Alpha (s) Signalling Events Pathway (119549)

This gene encodes a polypeptide hormone precursor that undergoes extensive, tissue-specific, post-translational processing via cleavage by subtilisin-like enzymes known as prohormone convertases. There are eight potential cleavage sites within the polypeptide precursor and, depending on tissue type and the available convertases, processing may yield as many as ten biologically active peptides involved in diverse cellular functions. The encoded protein is synthesized mainly in corticotroph cells of the anterior pituitary where four cleavage sites are used; adrenocorticotrophin, essential for normal steroidogenesis and the maintenance of normal adrenal weight, and lipotropin beta are the major end products. In other tissues, including the hypothalamus, placenta, and epithelium, all cleavage sites may be used, giving rise to peptides with roles in pain and energy homeostasis, melanocyte stimulation, and immune modulation. These include several distinct melanotropins, lipotropins, and endorphins that are contained within the adrenocorticotrophin and beta-lipotropin peptides. Mutations in this gene have been associated with early onset obesity, adrenal insufficiency, and red hair pigmentation. Alternatively spliced transcript variants encoding the same protein have been described. [provided by RefSeq, Jul 2008]

POMC: ACTH stimulates the adrenal glands to release cortisol. Defects in POMC may be associated with susceptibility to obesity (OBESITY). It is a condition characterized by an increase of body weight beyond the limitation of skeletal and physical requirements, as the result of excessive accumulation of body fat. Defects in POMC are the cause of pro-opiomelanocortinin deficiency (POMCD). Affected individuals present early-onset obesity, adrenal insufficiency and red hair. Belongs to the POMC family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 2p23.3. Cellular Component: peroxisomal matrix; extracellular space; cytoplasm; extracellular region; peroxisome; secretory granule. Molecular Function: type 3 melanocortin receptor binding; G-protein-coupled receptor binding; hormone activity; type 4 melanocortin receptor binding; receptor binding. Biological Process: generation of precursor metabolites and energy; cellular protein metabolic process; cell-cell signaling; neuropeptide signaling pathway; regulation of blood pressure; regulation of appetite; peptide hormone processing; positive regulation of transcription from RNA polymerase II promoter; negative regulation of tumor necrosis factor production; signal transduction; glucose homeostasis; cellular pigmentation. Disease: Obesity; Proopiomelanocortin Deficiency

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA & Azide at 1mg/ml)

340