CD86 | MyBioSource MBS438023 product information

product summary

company name :

MyBioSource

product type :

antibody

product name :

CD86

catalog :

MBS438023

quantity :

0.5 mL

price :

340 USD

clonality :

monoclonal

host :

mouse

conjugate :

nonconjugated

clone name :

[BU63]

reactivity :

human, mouse, rat

application :

immunocytochemistry, flow cytometry, FACS

more info or order :

MyBioSource product webpage

image

image 1 :

FCM staining of human PBMCs using CD86 MAb (BU63).

product information

catalog number :

MBS438023

products type :

Antibody

products full name :

CD86

products short name :

[CD86]

products name syn :

[Activation B7-2 antigen; B lymphocyte activation antigen B72; CD28 antigen ligand 2; CD28LG2; CLS1; CTLA-4 counter-receptor B7.2; Early T-cell costimulatory molecule 1; ETC-1; FUN-1; LAB72; Ly-58; MB7-2; TS/A-2]

other names :

[T-lymphocyte activation antigen CD86 isoform 4; T-lymphocyte activation antigen CD86; T-lymphocyte activation antigen CD86; BU63; FUN-1; CTLA-4 counter-receptor B7.2; B-lymphocyte activation antigen B7-2; CD86 antigen (CD28 antigen ligand 2, B7-2 antigen); CD86 molecule; Activation B7-2 antigen; B70; BU63; CTLA-4 counter-receptor B7.2; FUN-1; CD_antigen: CD86]

products gene name :

[CD86]

other gene names :

[CD86; CD86; B70; B7-2; B7.2; LAB72; CD28LG2; CD28LG2]

uniprot entry name :

CD86_HUMAN

clonality :

Monoclonal

isotype :

IgG1, kappa

clone :

[BU63]

host :

Mouse

reactivity :

Human, Mouse, Rat.

sequence length :

217

specificity :

Recognizes a protein of 70kDa, which is identified as CD86 (HLDA V; WS Code BP BP072. HLDA V; WS Code A A109. HLDA VI; WS Code BP 95. HLDA VI; WS Code B CD86.9). CD86 is a type I transmembrane glycoprotein and a member of the immunoglobulin superfamily of cell surface receptors. It is expressed at high levels on resting peripheral monocytes and dendritic cells and at very low density on resting B and T lymphocytes. CD86 expression is rapidly upregulated by B cell specific stimuli with peak expression at 18 to 42 hours after stimulation. CD86, along with CD80/B71, is an important accessory molecule in T cell co-stimulation via its interaction with CD28 and CD152/CTLA4. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. It is also found on malignant Hodgkin and Reed Sternberg (HRS) cells in Hodgkin's disease.

purity :

Purified Ab conjugated to PE

form :

200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.

storage stability :

Store at 2 to 8 degree C. Antibody is stable for 24 months.

tested application :

Flow Cytometry (FC/FACS), Immuofluorescence (IF)

app notes :

Flow Cytometry: 5ul per test per one million cells in 0.1ml (or 5ul per 100ul of whole blood). Immunofluorescence (1:50-1:100)v Optimal dilution for a specific application should be determined.

image1 heading :

Testing Data

other info1 :

Localization: Cell surface. Positive Control: 293T or Jukat cells. Monocytes and dendritic cells, activated T, B and natural killer cells in lymph node or tonsil. Human Chromosomal Location: 3q13.33. Unigene: 171182 (Human)

other info2 :

Immunogen: ARH-77 (B-lymphoblastoid cell line). Quantity and Buffer: 0.5 ml at 200ug/ml; Conjugated to PE

products references :

1. Engel P, Gribben JG, Freeman GJ, Zhou LJ, Nozawa Y, Abe M, Nadler LM, Wakasa H, Tedder TF: The B7-2 (B70) costimulatory molecule expressed by monocytes and activated B lymphocytes is the CD86 differentiation antigen. Blood. 1994;84(5):1402-7. 2. Caux C, Vanbervliet B, Massacrier C, Azuma M, Okumura K, Lanier LL, Banchereau J: B70/B7-2 is identical to CD86 and is the major functional ligand for CD28 expressed on human dendritic cells. J Exp Med. 1994;180(5):1841-7. 3. Mauri D, Wyss-Coray T, Gallati H, Pichler WJ: Antigen-presenting T cells induce the development of cytotoxic CD4+ T cells. I. Involvement of the CD80-CD28 adhesion molecules. J Immunol. 1995;155(1):118-27. 4. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). 5. Leukocyte Typing VI., Kishimoto T. et al. (Eds.), Garland Publishing Inc. (1997).

ncbi gi num :

332634950

ncbi acc num :

NP_001193853.1

ncbi gb acc num :

NM_001206924.1

uniprot acc num :

P42081

ncbi mol weight :

70kDa

ncbi pathways :

Adaptive Immune System Pathway (366160); Allograft Rejection Pathway (920963); Allograft Rejection Pathway (83123); Allograft Rejection Pathway (535); Autoimmune Thyroid Disease Pathway (83121); Autoimmune Thyroid Disease Pathway (533); CD28 Co-stimulation Pathway (119553); CD28 Dependent PI3K/Akt Signaling Pathway (119554); CD28 Dependent Vav1 Pathway (119555); CTLA4 Inhibitory Signaling Pathway (119556)

ncbi summary :

This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]

uniprot summary :

CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Immunoglobulin superfamily; Membrane protein, integral. Chromosomal Location of Human Ortholog: 3q21. Cellular Component: cell surface; intracellular membrane-bound organelle; plasma membrane; integral to membrane; external side of plasma membrane. Molecular Function: protein binding; coreceptor activity; receptor activity; receptor binding. Biological Process: positive regulation of lymphotoxin A biosynthetic process; viral reproduction; T cell activation; negative regulation of T cell anergy; nerve growth factor receptor signaling pathway; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; myeloid dendritic cell differentiation; positive regulation of activated T cell proliferation; positive regulation of interleukin-4 biosynthetic process; positive regulation of T-helper 2 cell differentiation; cell-cell signaling; T cell proliferation during immune response; positive regulation of cell proliferation; response to yeast; defense response to virus; aging; response to drug; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; B cell activation; T cell costimulation; toll-like receptor signaling pathway; innate immune response; immune response

size1 :

0.5 mL

price1 :

340 USD

more info or order :

MyBioSource product webpage