antibody

CD3e (T-Cell Marker) Mouse Monoclonal Antibody [Clone C3e/1308]

MBS4380150

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

monoclonal

mouse

nonconjugated

[C3e/1308]

western blot, immunohistochemistry, immunocytochemistry, flow cytometry, FACS

Antibody

CD3e (T-Cell Marker) Mouse Monoclonal Antibody [Clone C3e/1308]

[CD3e (T-Cell Marker)]

[CD 3E; CD3 epsilon; CD3 TCR complex; CD3E; CD3e antigen epsilon polypeptide (TiT3 complex); T cell antigen receptor complex epsilon subunit of T3; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 epsilon chain; T3E; TCRE; TiT3 complex]

[CD3e]

Monoclonal

IgG2b, kappa

[C3e/1308]

Mouse

Human

Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

200ug/ml of Ab purified from rabbit anti-serum by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.

Antibody with azide - store at 2 to 8 degree C. Antibody without azide - store at -20 to -80 degree C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Flow Cytometry (FC/FACS), Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)

Flow Cytometry (1-2ug/million cells); Western Blotting (1-2ug/ml for 60 minutes at RT). Optimal dilution for a specific application shoe be determined.

Western Blot (WB)

SDS-PAGE

Western Blot

Flow Cytometric

Flow Cytometric Analysis of Jurkat cells using CD3e Monoclonal Antibody (C3e/1308) followed by Goat anti-mouse IgG-CF488(Blue); Isotype Control (Red).

Immunogen: Recombinant human CD3e fragment (aa 23-119). Human Chromosome Location: 11q23.3

Cellular Localization: Cell surface and cytoplasmic. Positive Control: Jurkat cells. Tonsil and lymph node

Cibull ML et. al. Histopathology, 1989, 15(6):599-605. Mason DY et. al. Journal of Clinical Pathology, 1989, 42(11):1194-200.

P07766

20kDa

The CD3 complex mediates signal transduction, resulting in T-cell activation and proliferation. Required for normal immune responses (PubMed:15546002, PubMed:8490660).

0.02 mg (With BSA & Azide at 0.2 mg/ml)

190 USD

0.1 mg (With BSA & Azide at 0.2mg/ml)

340

0.1 mg (Without BSA at 1mg/ml)

340